HPLC法测定二维亚铁颗粒中维生素C和维生素B_1的含量

Quantitative determination of vitamin C and vitamin B_1 in ferrous fumarate granules by HPLC

目的建立同时测定二维亚铁颗粒中维生素C和维生素B1含量的HPLC方法。方法采用Insteril ODS-3色谱柱，流动相为0．03mol／L磷酸氢二铵溶液（加入0．01％庚烷磺酸钠，用磷酸调节pH至3．0）-甲醇，梯度洗脱，检测波长为244nm，流速为0．6～1．0ml／min，柱温30℃。结果维生素C与维生素B1分别在0．501～6．011μg（r=1．0000）与0．038～0．456μg（r=0．9999）范围内线性关系良好，平均回收率分别为99．51％与99．20％，RSD分别为0．55％与0．49％（n=9）。结论本法简便、结果准确可靠，可用于二维亚铁颗粒中维生素c和维生素B，的质量控制。

Objective To establish a method for the simultaneous determination of vitamin C and vitamin B1 in ferrous fumar- ate granules. Methods The procedure was performed on the Insteril ODS-3 Cls column at 30 ℃ , detected at 244 nm. The mobile phase was composed by a gradient of 0. 03 mol/L of ammonium phosphate solution （in which 0.01% of sodium heptanesulfonate was added and the pH was adjusted to 3.0 by phosphoric acid） and methanol, with the flux at 0.6 - 1.0 ml/min. Results Either vitamin C or vitamin Bj was in good linear at 0. 501 - 6.011 μg, r = 1. 0000 and 0. 038 - 0. 456μg, r = 0. 999 9, respectively. The mean re- coveries were 99.51% and 99.20% , with the RSD O. 55% and 0.49% , n = 9. Conclusion The method was proved to be simple, accurate and reliable, appropriate for the quality control for vitamin C and vitamin B1 in ferrous fumarate granules.