脑源性神经营养因子在视网膜神经细胞bcl-2蛋白动态表达中的作用

Function of brain-derived neurotrophic factor in dynamic Bcl-2 protein expression of retinal nerve cells

目的探讨脑源性神经营养因子在视网膜神经细胞bcl-2蛋白动态表达中的作用。方法选择新生小牛视网膜神经细胞进行传代培养,当传至第2代时随机分为观察组及对照组,两组神经细胞个数相同,观察组在接种后的第3天加入脑源性神经营养因子(浓度为50μg/L),对照组不加入任何营养因子。分别于给药后的第2、4、6天应用Western blotting法检测两组视网膜神经细胞中bcl-2蛋白的表达情况。结果观察组给药后第2天bcl-2蛋白表达灰度值为0.451±0.049、给药后第4天bcl-2蛋白表达灰度值为0.376±0.043、体外培养1个月神经元细胞存活数为13.10±1.35、NSE阳性表达细胞数10.53±1.12,均显著高于对照组,差异有统计学意义(P<0.05);给药后第6天bcl-2蛋白表达灰度值为0.291±0.037,与对照组比较,差异无统计学意义(P>0.05)。结论在短时间内,脑源性神经营养因子对视网膜神经细胞bcl-2蛋白的表达具有促进作用,能够在一定意义上保护视网膜神经元细胞。

Objective To discuss the function of brain-derived neurotrophic factor （bdnf） in the dynamic Bcl-2 protein expression of retinal nerve cells. Methods The neonatal calf retinal neurons were selected for subculture. They were randomly divided into two groups at the second generation. Both groups with the same number of nerve cells, bdnf （50 μg/L） was added on the third day after vaccination in observation group, and there was not any neurotropbic factor for the control group. We used Western blotting method to detect Bcl-2 protein expression of retinal neurons on the second, fourth and sixth day after giving the agents respectively. Results In observation group, the grayscale of Bcl-2 protein expression was 0.451 ± 0. 049 for two days after giving the drugs, and that of Bel-2 protein expression was 0. 376 ± 0. 043 for four days after giving the drugs. The survival number of the neuronal cell was 13.10 ± 1.35 for one month in vitro culture, and the number of NSE-positive cells was 10.53 ± 1.12. They were significantly higher than that of the control group （P 〈 0.05 ）. The grayscale of Bcl-2 protein expression was 0. 291 ± 0. 037 for six days after giving the drugs, not significantly different from that of the control group （ P 〉 0.05 ）. Conclusion It is suggested that bdnf has catalytic function for dynamic Bel-2 protein expression of retinal nerve cells within a short duration. It can protect the retinal neuron cells in a sense.