芍药甘草汤对慢传输型便秘大鼠肠道运动及血浆胃肠激素的实验研究

Study of peony and licorice decoction on colonic myoelectricity and plasma gastrointestinal hormone of slow-transit constipation in rats

目的探讨芍药甘草汤对慢传输型便秘大鼠肠道动力的作用及机制。方法将36只SD大鼠随机均分为正常对照组,大黄模型对照组(大黄模型组)和大黄芍药甘草汤组(实验组),用大黄复制泻剂结肠模型并用芍药甘草汤进行干预,观察活性炭在肠道内推进的长度,计算活性炭推进长度占肠道全长的百分比,同时用放射免疫分析法测定大鼠血浆胃肠激素P物质(SP)和血管活性肠肽(VIP)的水平。结果大黄模型组较正常对照组肠管显著延长(P<0.01),实验组较大黄模型组肠管显著缩短(P<0.01);活性炭推进长度占肠道全长的百分比:大黄模型组较正常对照组显著降低(P<0.01);实验组较大黄模型组显著增高(P<0.01);大黄模型组与正常对照组比较,血浆SP含量显著降低(P<0.05),VIP显著增高(P<0.05);实验组与大黄模型组比较,SP含量显著增高,VIP显著下降(P<0.01)。结论大黄可以使肠管延长;芍药甘草汤可以缩短肠管,并通过调节SP和VIP的释放,改善肠道功能。

Objective To study the effects of peony and licorice decoction （PLD） on colonic myoelectricity of slow-transit constipa- tion in rats and their mechanism. Methods A total of 36 Sprague Dawley rats were randomly assigned into three groups： rhubarb group, PLD group and control group. The cathartic colon model was established by rhubarb, and intervened by peony and licorice decoction. Intestinal transit function was detected by activated carbon pushing test and plasma levels of substance P（SP） and vasoae-rive intestinal peptide （VIP） were tested after treatment. Results The intestinal transit function of rhubarb group showed a signifi- cant decrease when compared with that of control group （ P 〈 0.01 ） , and that of PLD group was significantly higher than that of rhubarb group （ P 〈 0.05 ）. The plasma level of SP was significantly lower, while that of VIP was significantly higher, in rhubarb group than in control group （P 〈 0.01 ）. The plasma level of SP was significantly higher, while that of VIP was significantly lower, in PLD group than in rhubarb group （ P 〈 0.01 ）. Conclusion PLD is effective on slow-transit constipation, and it can improve blood circulation and regulate the plasma levels of VIP and SP.