新兵流感病毒抗体检测的临床分析

Clinical Analysis of the Detection of Influenza Virus Antibody in Recruits

【目的】应用间接免疫荧光法（IFA）检测出现呼吸道症状且腋温≥37．3℃的新兵流感病毒A／B（FluA、B）抗体，探讨其快速检测FluA、B抗体的可行性，调查呼吸道感染且发热患者FluA、B抗体的水平。【方法】收集北京郊区某部队5个营区中出现咳嗽、咳痰、流涕、咽痛等呼吸道症状且腋温≥37．3。C的94名新兵的血清标本，应用IFA检测FluA、B抗体，分析检测结果。【结果]FluA、BIgM抗体阳性率分别为2，1％、8．5％；北方籍出现呼吸道症状且发热的新兵人数明显高于南方籍新兵、沿海明显高于内陆，差异均有统计学意义（P〈0．05），各营区间差异无统计学意义（P〉0．05）；南北方籍、沿海和内陆、各营区间新兵恢复期FluBIgM抗体差异均无统计学意义（P〉0．05）。【结论】流感病毒感染不容忽视，IFA法检测操作简单、便捷，适合在流感病毒流行时大规模快速筛查患者。

[Objective]To detect the antibody level of influenza virus A（Flu A） and B（Flu B） in recruits with respiratory symptoms and axillary temperature ~ 37.3~C by indirect immunofluorescence assay（IFA）, and to explore the feasibility of rapid detection of Flu A and B antibodies, and to investigate the level of Flu A and B antibodies in patients with respiratory infections and fever. [Methods] Serum samples were collected from 94 recruits with cough, sputum, runny nose, sore throat and other respiratory symptoms and axillary temperatureS37.3℃ in five camps around Beijing, Flu A and B antibodies were detected by IFA. The test re- sults were analyzed. [Results] Positive rate of IgM antibody of Flu A and B were 2.1% and 8.5 %, respec- tively. The northern recruits with respiratory symptoms and fever were significantly higher than southern re- cruits, and recruits in coastal areas were significantly higher than those in inland areas, and there were signifi- cant differences（ P 〈0.05）, but there was no significant difference between different military camps. There was no significant difference in IgM antibody of Flu B in recruits during recovery period in northern, southern, coastal and inland areas and different military camps（ P 〉0.05）. [Conclusion]Influenza virus infection can not be ignored. IFA method is simple, convenient and suitable for rapid screening of patients in the epidemics of influenza virus.