摘要
从绳状青霉(Penicillium funiculosum)深层发酵液中分离得到一种未知的β-葡萄糖苷酶,并对其酶学性质进行初步分析。粗酶液经硫酸铵沉淀、DEAE-Sephadex A-25离子交换层析、Sephadex G-50凝胶过滤层析等步骤,获得了一种凝胶电泳均一的β-葡萄糖苷酶,经SDS-PAGE测得其分子量为19kDa,其最适反应温度为60℃、最适反应pH值为4.5。
A β-glucosidase was purified and prelimarily characterized from submerged fermentation of Peni- cilliurnfuniculosum. The β-glucosidase was purified by ammonium sulfate precipitation, DEAE-Sephadex A-25 anion-exchange chromatography and Sephadex G-50 gel-filtration chromatography. SDS-PAGE Showed the pu- rified enzyme to be a homogeneous protein with molecular weight of 19 kDa. Its optimal reaction temperature and pH value were 60 ℃ and 4.5,respectively.
出处
《化学与生物工程》
CAS
2013年第10期38-41,共4页
Chemistry & Bioengineering
基金
东北农业大学大学生创新团队发展计划项目(CXT 003-2-1)
关键词
绳状青霉
Β-葡萄糖苷酶
纯化
酶学性质
Penicilliumfuniculosum ^-g｜ucosidase^purification
enzymatic property
