Oct4在宫颈癌中的表达及与人乳头瘤病毒感染的关系

Expression of Oct4 in cervical carcinoma and its association with HPV infection

目的探讨宫颈癌细胞中干细胞关键转录因子Oct4的表达情况及意义,并探讨Oct4表达与人乳头瘤病毒感染的关系。方法采用免疫组织化学法和反转录-聚合酶链反应(RT-PCR)检测20例宫颈癌组织、30例宫颈上皮内瘤变(CIN)组织及20例正常宫颈组织标本中Oct4的表达及PCR检测所有标本中人乳头瘤病毒(HPV)16/18DNA感染情况。结果宫颈癌组织Oct4阳性表达率为90%(18/20),CIN中为50%,和正常宫颈组织中相比两者差异均具有统计学意义。而且宫颈癌组织中Oct4的表达不仅与其肿瘤分化程度密切相关,与HPV感染也有关,表现为HPV16/18DNA阳性个体中Oct4阳性率高,而HPV16/18DNA阴性个体中Oct4阳性率低。结论联合检测Oct4和高危型HPV DNA可作为宫颈癌一个敏感性、特异性的标志和治疗靶点。

Objective To investigate the expression and the correlation of Oct4 and human papilloma virus （HPV） in cervical carcinoma .Methods Immunohistochemical analysis and reverse transcript polymerase chain reac-tion（RT-PCT ） were used to detect the expression of Oct4 in tissue samples from 20 cases of cervical carcinoma ,30 cases of cervical intraepithelial neoplasia （CIN ） and 20 cases of normal cervix .HPV 16/18 DNA was detected by PCR .Results The positive rates of Oct4 in cervical carcinoma was 90% （18/20） ,obviously higher than CIN （50% ） and normal cases（5% ） .Moreover ,the expression of Oct4 in cervical carcinoma was also associated with poor differ-entiation and HPV16/18 infection .The positive rate of Oct4 in HPV16/18 infective patients was higher than patients without HPV16/18 infection .Conclusion High expression of Oct4 in cervical carcinoma might be associated with the degree of differentiation and HPV16/18 infection ,which could be an useful target for diagnosis and treatment of cer-vical carcinoma .