九香虫防御性物质水溶液成分分析及其对LO2细胞活性的影响

Analysis of the Chemical Constituents of Defensive Substances from Aspongopus chinensis and their Effect on the Activity of LO2 Cells

研究了九香虫的防御性物质水溶液中挥发性成分及其相对百分含量,以及该水溶液对人肝LO2细胞活性的研究。采用温水浸泡法获得九香虫的防御性物质水溶液;GC-MS获得该水溶液的挥发性成分组成,并用面积归一法计算各成分的相对百分含量;利用MTT法、流式细胞术研究该水溶液对LO2细胞的影响。研究表明,九香虫防御性物质水溶液中挥发性成分包含19种化合物,其中相对百分含量最高的为反-2-己烯醛(88.54%),其次为2-甲基-4-戊烯醛(5.16%)、十三烷(3.43%)等;该水溶液作用LO2细胞后,IC50为3.64μL,呈剂量依赖性;细胞周期结果显示,G2/M期细胞比例明显降低,而G0/G1期细胞比例明显升高,且差异具有统计学意义。九香虫防御性物质水溶液成分主要是烯醛和烷烃类化合物;该物质能够抑制人肝LO2细胞的体外增殖,可能与其细胞周期阻滞有关。

The volatile components and their relative contents of the defensive substances in aqueous solution from Aspongopus chinensis, and the effect of aqueous solution on the activity of normal human liver LO2 cells were studied in this research.Warm water immersion of bugs was used to obtain the defensive substances solution, and GC-MS and area normalization was employed to identify the volatile components and calculate their relative contents. MTT and flow cytometry was used to find the effect of the defensive substances solution on liver LO2 cell. The research showed that the solution contained 19 kinds of chemical compounds, and the anti -2- hexenal had the highest relative percentage content 88.54%, then followed by 2-methyl-4-pentene aldehyde （5.16%） and trudecyl （3.43%）; IC50 was 3.64 μL which presented dose-dependent manner after the solutions reacted with LO2 cells. The result of flow cytometry showed that, compared with the control group, the percentage of G2/M phase cells from the drug group were reduced obviously and G0/G1 phase cells were increased significantly. The major compounds of Aspongopus chinensis defensive substances solution were enal and alkanes, which could inhibit the proliferation of normal human liver LO2 cells in vitro because of cell cycle arrest.