摘要
本文利用岩藻聚糖硫酸酯降解酶(EQFF)对大分子量岩藻聚糖硫酸酯进行降解,进而得到低分子量的酶解产物,比较并分析了酶解前后岩藻聚糖硫酸酯对D-半乳糖氧化损伤小鼠的抗氧化作用。将SPF级昆明种雄性小鼠随机分为正常对照组、模型组、岩藻聚糖硫酸酯组、酶解物组、阳性对照组,饲养42 d后处死,测定血清和肝脏组织的抗氧化指标。试验结果表明,岩藻聚糖硫酸酯及其酶解产物与模型组相比都具有显著的抗氧化活性。岩藻聚糖硫酸酯酶解物组小鼠抑制血清和肝脏组织的·OH的活性达到500.67±35.67 U/mL和1821.34±162.02 U/mg prot,T-SOD活力达到101.82±3.98 U/mL和3053.36±245.88 U/mg prot;同时,岩藻聚糖硫酸酯酶解物组小鼠的血清CAT活力达到4.05±0.56 U/mL,GSH-Px活力达到348.88±29.22 U,说明低分子量的酶解产物比高分子量的岩藻聚糖硫酸酯有更显著的抗氧化作用,为岩藻聚糖硫酸酯的应用提供了依据。
To investigate the antioxidant effects of fucoidan and its hydrolysates on D-galactose-induced senescent mice, hydrolysates of Fucoidan were prepared by EQFF degradation from high molecular weight Fucoidan. Kunming male mice were randomly divided into five groups: normal control group, oxidative injury model group, fucoidan group, hydrolysates of fucoidan group, and positive control group. Each group was injected with different drugs at the same dose for successive forty-two days. Then the indices of antioxidant effects both in serum and liver were determined. The results showed that both fucoidan and its hydrolysates had significant antioxidant effects compared with the model group. The inhibiting effects of fucoidan hydrolysates on hydroxyl radical of the mice feeding reached 500.67±35.67 U/mL (serum) and 1821.34±162.02 U/mgprot (liver), and the activity of T-SOD achieved were 101.82±3.98 U/mL (serum) and 3053.36±245.88 U/mgprot (liver). Meanwhile, the activity of CAT and GSH-Px in serum reached 4.05±0.56 U/mL and 348.88±29.22 U,respectively. It was indicated that the low-molecular- weight fucoidan hydrolysates had higher antioxidant influence on D-galactose-induced senescent mice than high- molecular- weight fucoidan.
出处
《现代食品科技》
EI
CAS
北大核心
2013年第10期2378-2382,共5页
Modern Food Science and Technology
基金
国家自然科学基金资助项目(30800858)