胃蛋白酶原Ⅱ的检测与应用

Detection of pepsinogenⅡby three different methods and its clinical application

目的探讨时间分辨荧光法(TRFIA)、化学发光免疫分析法(CLIA)和酶联免疫吸附法(ELISA)检测胃蛋白酶原Ⅱ(PGⅡ)的相关性,及其临床实用性。方法在TRFIA法检测的临床标本中随机选择88份血清标本,分别用CLIA法和ELISA法再次检测PGⅡ,分析3种方法检测结果的相关性;对消化科门诊/病房患者和体检人群进行回顾性分析,验证TRFIA法检测PGⅡ对胃病筛查的可行性。结果 TRFIA法(Y)与CLIA法(X)PGⅡ检测结果呈显著正相关(r=0.946 7,P=0.000 1),回归方程为:Y=0.952 8 X+0.571 1(P=0.000 1);TRFIA法(Y)与ELISA法(X)PGⅡ检测结果呈显著正相关(r=0.961 8,P=0.000 1),回归方程为:Y=0.727 5 X+0.2617(P=0.0001);ELISA法(Y)与CLIA法(X)PGⅡ检测结果呈显著正相关(r=0.980 6,P=0.000 1),回归方程为:Y=1.304 9 X+0.480 0(P=0.000 1);体检人群、消化科门诊患者、消化科住院患者的血清PGⅡ水平分别为(15.97±10.82)ng/mL、(20.75±17.27)ng/mL、(35.64±41.14)ng/mL,消化科门诊患者血清PGⅡ水平明显高于体检人群(P<0.05),消化科住院患者血清PGⅡ水平明显高于消化科门诊患者(P<0.05)。结论 PGⅡ可能用于浅表性胃炎、胃十二指肠糜烂或溃疡的筛查,但鉴于不同方法检测结果的差异,建议各实验室根据不同方法建立自己的参考值。

Objective To evaluate the correlation of three pepsinogenⅡ（PGⅡ）detection methods and its clinical applications.Methods 88serum samples were randomly selected,and PGⅡwas detected by using time-resolved fluoroimmunoassay（TRFIA）,chemiluminescence immunoassay（CLIA）and enzyme-linked immunosorbent assay（ELISA）,respectively.Then,their results were processed using regression and correlation analysis.To verify the feasibility of gastric disease screening by PGⅡdetection,5 439 people were divided into three groups：gastroenterology outpatients,gastroenterology hospitalized patients,and physical examination population.Results It showed a significant positive correlation between any two of the three methods（P＆lt;0.05）.PGⅡserum levels were（15.97±10.82）ng/mL,（20.75±17.27）ng/mL,and（35.64±41.14）ng/mL in physical examination population,outpatients,and hospitalized patients,respectively.There was significantly difference between the three groups（P＆lt;0.05）.Conclusion PGⅡdetection may be used for the screening of superficial gastritis and gastroduodenal ulcer.But the laboratory should establish its own reference values according to different methods.