乙型肝炎、肝硬化和肝癌患者RORγtmRNA表达的研究

The study of PBMC RORγt mRNA expression in patients with chronic hepatitis B,cirrhosis and liver cancer

目的研究乙型肝炎、肝硬化和肝癌患者外周血单个核细胞Th17细胞特异转录因子———RORγt的mRNA表达。方法采用实时定量RT-PCR测定乙型肝炎、肝硬化和肝癌患者外周血单个核细胞RORγt基因mRNA表达。结果健康对照组外周血单个核细胞RORγt mRNA表达为0.31±0.13,乙型肝炎组为1.23±0.25,肝硬化组为1.36±0.21,肝癌组为1.42±0.27。乙型肝炎、肝硬化和肝癌组外周血单个核细胞RORγt mRNA表达与健康对照组比较,差异均有统计学意义(P<0.01)。结论乙型肝炎、肝硬化和肝癌患者外周血单个核细胞RORγt mRNA表达升高,RORγt在乙型肝炎、肝硬化和肝癌的发病中具有重要的作用。

Objective To study peripheral blood mononuclear cells Th17cell specific transcription factor RORγt mRNA expression in patients with chronic hepatitis B,cirrhosis and liver cancer.Methods PBMC RORγt mRNA expression in patients with chronic hepatitis B,cirrhosis and liver cancer were measured with real time RT-PCR.Results Healthy control group PBMC RORγ t mRNA expression was 0.31±0.13,hepatitis B group was 1.23±0.25,liver cirrhosis group was 1.36±0.21,liver cancer group was 1.42±0.27.Compared with health control group,hepatitis B,liver cirrhosis and liver cancer group PBMC RORγt mRNA expression have significant differences（P＆lt;0.01）.Conclusion PBMC mRNAγt expression increased in patients with chronic hepatitis B,liver cirrhosis and liver cancer,RORγt plays an important role in the pathogenesis of hepatitis B,liver cirrhosis and liver cancer.