高湿环境下血清胸苷激酶1水平的变化

Changes of serum thymidine kinase 1 levels in different humidity

目的通过对血清中胸苷激酶1(TK-1)含量检测分析,了解高湿环境下细胞增殖变化情况,研究高湿环境与肿瘤之间的关系。方法应用酶联免疫印迹化学发光法检测在高湿环境下饲养20d的SD大鼠(10只),饲养60d的SD大鼠(10只)血清TK-1水平含量。结果 60d饲养组TK-1血清水平为(3.8±2.66)PM/L高于20d饲养组(1.45±0.45)PM/L,差异有统计学意义(t=2.76,P<0.05);两组TK-1血清水平比较差异有统计学意义(P<0.05)。结论处于高湿环境中能够对SD大鼠的细胞增殖过程产生影响,且延长处于高湿环境时间这样的影响将更加明显。

Objective To find out the change of cell proliferation in the highly humid environment and explore the relationship between tumor and the humid environment,by testing the content of serum thymidine kinase 1（TK-1）.Methods 20 SD rats were divided into two groups（n=10）,ones were fed 20 days in the highly humid environment,the others were fed 60 days in the same environment.The enzyme-linked immunoblotting chemiluminescence method was applied to detect the level of the serum thymidine kinase 1 in the sample rats.T-test was used to analyze the difference of the two groups.Results Serum TK-1 level of the latter was higher than that of the former[（3.8±2.66）PM/L）.vs.（1.45±0.45PM/L）]（t=2.76,P＆lt;0.05）.There were significant differences of the serum TK-1 levels in the two groups（P＆lt;0.05）.Conclusion Humidity exerts profound effect on the cell proliferation.The effect can be more obvious when they are exposed in such environment in the long run.