肺炎支原体不同检测方法的比对分析

Comparison of different diagnostic methods for mycoplasma pneumoniae antibody

目的评价被动凝集法和间接免疫荧光法（IIFA）在肺炎支原体（MP）抗体检测中的特点及应用价值。方法收集2011年8~12月儿科住院怀疑MP感染患者血清170例,采用IIFA和被动凝集法对标本进行检测,比较两种方法的灵敏度和特异性。结果 IIFA灵敏度72.8%,被动凝集法灵敏度93.6%,二者比较差异有统计学意义（P〈0.01）;IIFA特异度97.7%,被动凝集法特异度64.4%,二者比较差异有统计学意义（P〈0.01）;IIFA诊断符合率79.4%,被动凝集法诊断符合率85.9%,二者比较差异无统计学意义（P〉0.05）。结论应联合应用两种方法可提高MP感染诊断的正确性。

Objective To compare test characteristic of IIFA and particle agglutination test for MP antibody detecting and evaluate their values.Methods MP antibody were detected in 170 serum samples from MP Pneumonia or MP pneumonia suspicious patients by IIFA and particle agglutination test separately.Results The sensitivity for IIFA and particle agglutination test were 72.8% and 93.6%,respectively （P＆lt;0.05）.And specificity for IIFA and particle agglutination test were 97.7% and 64.4%,respectively,the diffevences was staically significant （P＆lt;0.05）.Conclusion Canbined the two wethods can improve MP detecting specificity and sensitivity.