2011~2012年辽宁地区猪繁殖与呼吸综合征病毒NSP2基因遗传演化分析

NSP2 genetic evolution analysis of porcine reproductive and respiratory syndrome virus in swine herds in Liaoning Province from 2011 to 2012 years

本研究应用分子生物学技术,扩增并测定了2011~2012年辽宁地区6株PRRSV NSP2基因全序列。结果发现,6株PRRSV的NSP2基因全长均为2 850 bp,均于1 143~1 441 bp和1 593~1689 bp发生连续的碱基缺失,且均为变异毒株,具有典型HP-PRRSV的分子特征;同源性与进化分析表明,HP-PRRS在辽宁地区有不同的毒株同时流行,辽宁地区HP-PRRSV可能起源于同一祖先。

In this study, molecular biology techniques were used to amplify and se-quencing six strains PRRSV NSP2 genome sequences in swine herds in Liaoning Prov-ince from 2011 to 2012 years. It was found that full-length of six strains PRRSV NSP2 genome sequences were all 2 850 bp. continuous base deletion occurred at 1 441bp^1 143bp and 1 593bp^1 689bp of the six strains PRRSV NSP2 genome;and they were all variant strains. All of he six strains PRRSV NSP2 genome had typical HP-PRRSV molecular characteristics.The analysis of homology and phylogenetic showed that different strains of HP-PRRSV were prevalent in in swine herds in Liaon-ing Province at the same time. The strains of HP-PRRSV may originate from the same ancestor PRRSV strain in swine herds in Liaoning Province.