感冒胶囊含量测定的方法学考察与研究

Investigation and Study on the Content Determination Method of Cold Capsules

目的:制定简便、快捷的感冒胶囊含量测定的方法。方法:采用高效液相法测定了制剂中补骨脂素和异补骨脂素的含量。结果:通过方法学考察,补骨脂素的进样量在0.0393μg^0.393μg,异补骨脂素的进样量在0.0383μg^0.383μg,与峰面积呈良好的线性关系,补骨脂素的平均回收率为100.3%,RSD为0.8%,异补骨脂素的平均回收率为99.3%(n=6),RSD为0.7%.结论:制剂通过测定补骨脂素和异补骨脂素的含量,为感冒胶囊的质量控制提供了新的参考。

Objective：To establish a simple and convenient method for determining the content of cold capsules. Methods：By high performance liquid chromatography （HPLC）, the experiments assay the content of psoralen and isopsoralen in the cold capsules. Results： By the methodological study, when the injection volume of psoralen and isopsoralen are 0.0393 μ g-0.393μ g and 0.0383 μ g-0.383 μ g respectively, they showed a good linear relationship with the peak area. The average recovery and RSD of psoralen are 100.3% and 0.8%. The average recovery and RSD of isopsoralen are 99.3% and 0.7%. Conclusion：By determining the content of psoralen and isopsoralen in the cold capsules, provide a new reference for quality control of cold capsule.