马鞭草总黄酮对四氯化碳致小鼠急性肝损伤的影响

Protective Effect of Total Flavones of Herba Verbenae Against Carbon Tetrachlorideinduced Acute Liver Injury in Mice

目的研究马鞭草总黄酮对四氯化碳致小鼠急性肝损伤的治疗作用。方法 60只小鼠随机分成正常组,模型组,阳性对照组,马鞭草总黄酮低、中、高剂量组,每组10只。正常组和模型组灌胃给予等体积0.9%氯化钠溶液,马鞭草总黄酮低、中、高剂量组和阳性对照组分别灌胃给予马鞭草总黄酮(50,100,200 mg·kg-1)和联苯双酯(200 mg·kg-1),qd,连续10 d。末次给药1 h后,除正常对照组,其余各组腹腔注射0.15%四氧化碳(5 mL·kg-1),24h后测定血清中丙氨酸氨基转移酶(ALT),天冬氨酸氨基转移酶(AST)的活性和白细胞介素(IL)-6,肿瘤坏死因子(TNF)-α,IL-8的含量以及肝脏指数、脾脏指数、胸腺指数,检测肝组织核转录因子(NF-κB)p65的表达和超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px),过氧化氢酶(CAT)的活性,丙二醛(MDA),一氧化氮(NO)的含量。结果马鞭草总黄酮高、中剂量能降低血清中ALT、AST的活性(P<0.01),降低肝脏指数、脾脏指数、胸腺指数(P<0.05或P<0.01),抑制肝组织NF-κB p65的表达并降低血清中IL-6,TNF-α,IL-8的含量(P<0.05或P<0.01),升高肝组织中SOD,GSH-Px,CAT的活性,降低MDA,NO的含量(P<0.05或P<0.01)。结论马鞭草总黄酮对四氧化碳所造成的肝细胞的损伤具有较好的改善和保护作用,减少炎症的渗出,降低氧化损伤程度。

Objective To study the protective effect of total flavones of Herba verbenae （TFH） against carbon tetrachloride （CCl4）-induced acute liver injury in mice. Methods Sixty mice were randomly divided into six groups： normal group, model group, positive control group, high, medium, and low-dose TFH group. The mice of normal and model groups were given normal saline,and mice of all TFH groups and positive control group were administered with TFH （50,100,200 mg·kg^-1） and bifendate （200 mg·kg^-1） ,respectively. All the mice were treated with TFH or bifendate for 10 consecutive days,once daily. One hour after the last administration of the drugs, each mouse was intraperitoneally injected with 0. 15% CC14, except the mice in the normal group. Twenty four hours after the last administration of the drugs ,the activity of alanine aminotransferase （ALT）, aspartate aminotransferase （AST） , interleukin （IL） -6, tumor necrosis factor （TNF） -α, and interleukin （IL） -8 in the serum, liver index, spleen index,thymus index, the expression of nuclear transcription factor （ NF-KB ） p65, the activities of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px）, catalase （ CAT）, and the contents of malondialdehyde （MDA）, and nitric oxide （NO） in liver tissues were investigated. Results In high-and medium-dose TFH groups, the serum activities of ALT and AST were significantly decreased （ P〈0. 01 ）. The liver index, spleen index, thymus index were significantly reduced （ P〈0.05 or P〈0. 01 ）. The expression of NF-KB p65 was inhibited in the liver tissue, and the contents of IL-6, TNF-α, IL-8 were decreased in the serum （P〈0.05 or P〈0.01 ）. The activities of SOD, GSH-Px and CAT were elevated in the liver tissue, and the content of MDA, NO were reduced （P〈0. 05 or P〈0. 01 ）. Conclusion TFH can alleviate liver injury, protect liver cells, and reduce inflammatory exudation and oxidative injury.