鼻咽癌顺铂耐药细胞系的建立及其耐药性变化

Establishment of cis-Diammine-Dichloro-Platinum (cDDP)-resistant NPC Cell Lines (CNE2/cDDP) and Study of the Changes of Drug Resistance

目的通过顺铂(cDDP)浓度递增法建立鼻咽癌耐药的细胞株CNE2/cDDP,并研究其耐药性变化。方法采用cDDP浓度递增法建立鼻咽癌顺铂耐药细胞系CNE2/cDDP,倒置显微镜进行形态学观察,MTT检测CNE2/cDDP细胞系对不同化疗药物的耐药性。结果 MTT研究发现在不同药物(顺铂、长春新碱、卡铂、紫杉醇、5-FU)作用下,CNE2/cDDP较CNE2的IC50值及耐药指数均有明显的增加,CNE2/cDDP细胞对顺铂的耐药性最强,增加到200.6倍,对5-Fu的耐药性最差,增加到2.8倍;耐药细胞株CNE2/cDDP较非耐药细胞株CNE2生长速度减慢,其细胞倍增时间由18 h增加到24 h;CNE2/cDDP细胞体积较CNE2为小,常呈克隆聚集现象。结论CNE2/cDDP具有多药耐药性,是一个理想的用于鼻咽癌的多药耐药研究的细胞系。

Objective To study the changes of drug resistence of NPC by establishing the cis-Diammine-Dichloro- Platinum （eDDP） -resistant NPC cell lines （CNE2/eDDP） through eDDP concentration progressively increased method. Methods CNE2/cDDP was established by cDDP concentration progressively increased method. Morphology study was per- formed by invert microscope, and drug resistance to eDDP, Vincristin, Carboplatin, Taxotere, and 5-fluorouracil were detected by MTT. Results MTT showed that there was significant increase of IC50 and resistance index in CNE2/eDDP compared to CNE2, after dealt with cDDP, Vineristin, Carboplatin, Taxotere, and 5-fluorouracil. And the drug resistance of eDDP in CNE2/eDDP was the highest, and RI （resistance index） was increased to 200.6-folds compared to CNE2. While the lowest drug resistance was in 5-Fu, and RI was increased to 2.8-folds compared to CNE2. There were clone aggregation and slow growth speed in CNE2/cDDP and more longer doubling time increased from 18 h in CNE2/cDDP to 24 h in CNE2. And there were much smaller volume in CNE2/cDDP compared with CNE2. Conclusion CNE2/cDDP may serve as an ideal cell line used for study of drug resistance in NPC for its multi-drug resistance.