藻蓝素对脓毒症急性肺损伤大鼠的保护作用

Protective Effect of C-phycocyanin on Acute Lung Injury in Septic Rats

目的观察藻蓝素(CPC)对脓毒症急性肺损伤大鼠的保护作用。方法 SD大鼠随机分为对照组、模型组和CPC干预组。其中模型组采用盲肠结扎穿刺建立脓毒症急性肺损伤大鼠模型。CPC干预组在模型组基础上分别给予浓度为20 mg/kg、40 mg/kg和60 mg/kg CPC腹腔注射。术后72 h获取血液及肺组织标本,检测PaO2/FiO2、肺湿干重比、支气管肺泡灌洗液中肿瘤坏死因子α(TNF-α)、白细胞介素-1β(IL-1β)和IL-6水平,以及肺组织中丙二醛(MDA)水平及髓过氧化物酶(MPO)活性。结果与正常对照组相比,模型组PaO2/FiO2含量显著降低(P<0.05)。不同浓度CPC干预后,PaO2/FiO2进一步增高。模型组肺湿干重比以及MDA和MPO显著高于正常对照组(P<0.05),CPC处理后,肺湿干重、MDA和MPO水平随之降低;模型组大鼠BALF中TNF-α、IL-1β和IL-6含量明显增高,CPC能进一步降低其含量。结论 CPC可减轻脓毒症急性肺损伤大鼠肺部气体交换功能和炎症反应,从而发挥对脓毒症急性肺损伤大鼠的保护作用。

Objective To observe the protective effect of C-phycocyanin （CPC） on acute lung injury in septic rats. Methods SD rats were randomly divided into control group, model group and CPC group. Cecal ligation and puncture was used to establish septic acute lung injury rats （model group）. For the CPC groups,septic acute lung injury rats were ad- ministrated by 20,40 and 60mg/kg of CPC by peritoneal injection. 72 h after the operation,serum and lung tissue were ob- tained, the PaO2FiO2,the wet to dry weight ratio, content of TNF-α, IL-6 and IL-10 in bronchoalveolar lavage fluid, the concentration of malondialdehyde （MDA） and activity of myeloperoxidase （MPO） were analyzed. Results The PaO2/ FiO2 in the model group was lower than that in the control group （ P 〈 0. 05 ）, after CPC treatment, the level of PaOE/FiO2 increased. The wet to dry weight ratio and the content of MDA and activity of MPO in the model group was higher than that in the control group （P 〈 0. 05）, and were decreased in CPC groups. In the model group, TNF-α, IL-6 and IL-10 in bron- choalveolar lavage fluid was increased, and CPC could also descrease them as the concentration increased. Conclusion CPC could relieve gas exchange function and the inflammatory response, and thus play a protective role in the septic acute lung injury.