反义c-myc基因质粒对鼠脑胶质瘤细胞C6生长的影响

Influence of Antisense C-myc Gene Plasmid on Growth of Rat Glioma Cell C6

目的探讨反义ｃ－ｍｙｃ基因质粒对鼠脑胶质瘤细胞Ｃ６增殖及凋亡的作用。方法将可在真核细 胞表达大鼠反义 ｃ－ｍｙｃ基因的质粒 ｐＣＥＰ４ＡＳＣＭＲ以脂质体 ＦｕＧＥＮＥ６为介导，转染到大鼠脑恶性胶质瘤细胞 Ｃ６株 中，经ＭＴＴ检测，软琼脂集落生长，吖啶橙染色，琼脂糖电泳等方法分析实验结果。结果反义ｃ－ｍｙｃ基因质粒 ＤＮＡ具有抑制瘤细胞增殖的作用，并能引起瘤细胞凋亡；脂质体ＦｕＧＥＮＥ６有助于提高质粒ＤＮＡ的转染效率。结论 反义ｃ－ｍｙｃ基因质粒可抑制ｃ－ｍｙｃ基因的过度表达，促使肿瘤细胞凋亡，达到治疗脑神经胶质细胞瘤的目的。

Objective To study on the effect of antisense c-myc gene plasmid on the proliferation and apoptosis of rat glioma cell C6. Methods The plasmid pCEP4ASCMR expressing rat antisense c-myc gene in eukaryocyte was transfected to rat glioma C6 cell strain using Iiposome FuGENE6 as media, and the results were analyzed by MTT method, cell colony-forming test in soft agar medium and acridine orange staining. Results Antisense c-myc gene plasmid DNA could inhibit the proliferation and induce the apoptosis of glioma cells, and liposome FuGENE6 could promote the transfection of the plasmid DNA. Conclusion Antisense c-myc gene plasmid can inhibit the excessive expression of c-myc gene and is helpful to the treatment of glioma.