抗真菌蛋白质的分离纯化

Purification of Antifungal Protein from Aspergillus Niger

目的试图从霉菌培养液中分离纯化抗真菌活性蛋白质。方法用离子交换层析法分离，用反 向高效液相色谱法进一步纯化，经Ｎ－三（羟甲基）甲基甘氨酸凝胶电泳分析确定其相对分子质量。结果从黑曲霉 菌培养上清液中分离出一种具有抗真菌活性的蛋白质（ＡＦＰ）。纯化后得到高活性的精制ＡＦＰ。相对分子质量约为 ９０００，经ＭＴＴ法检测５种真菌具有明显抗真菌活性。结论本研究为抗菌药物及防腐剂的开发提供了依据。

Objective To purify the protein with antifungal activity from Aspergillus niger culture flu- id. Methods The protein was seperated by ion exchange column chromatography and further purified by reverse phase HPLC, and the molecular weight of it was determined by tricine gel electrophoresis. Results A kind of protein(AFP) with antifungal activity was seperated. The molecular weight of it was about 9 000. The detection result by MTT method showed significant antifungal activity of the protein to 5 kinds of fungi. Conclusion The study provided a reliable basis for developing drug and antiseptic.