应用异型双功能试剂连接制备乳胶妊娠诊断制剂

Preparation of Latex Diagnostic Kit for Pregnancy by Linking of Allotypic Bifunctional Reagents

目的研制稳定性特异性更好的妊娠诊断制剂。方法应用抗ＨＣＧ－β亚基Ｃ末端多肽抗体，以双 功能试剂连接到羧化苯乙烯乳胶上。结果制成的妊娠诊断试剂较目前通用的ＨＣＧ乳胶试剂灵敏度高，为１．５ＩＵ／ ｍｌ。稳定性好，在室温放置３个月，其灵敏度未下降。用本试剂检测１６０份妊娠尿和１５０份正常尿，结果准确率为 ９５％。结论本试剂所用方法优于通常应用的妊娠诊断方法。

Objective To prepare a stable and specific diagnostic kit for pregnancy Methods A diagnos- tic kit for pregnancy was prepared by linking of C - terminal polypeptide antibody of anti - HCG - subunit to car- boxyphenylethene latex with bifunctional reagents .Results Latex agglutination test showed that the sensitivity of the kit was 1.5 IU/ml. After being stored at room temperature for 3 months,no decrease of the sensitivity was ob- served. One huned sixty urine specimens from pregnant women and 150 normal urine were detected by the kit. The result showed that the accuracy rate of the kit was 95% .Conclusion The kit is superior to routine kit.