PVA-卡拉胶混合载体固定化大肠杆菌-酵母菌混合体系生产谷胱甘肽

Glutathione production by immobilized E.coli S.cerevisiae cells with PVA carrageenin

提出了PVA -卡拉胶混合载体固定化微生物细胞的技术 ,确定了较好的制备工艺 ,并用该载体对大肠杆菌 -酵母菌混合体系进行了固定化研究。结果表明 :在PVA浓度 1 0 % ,卡拉胶浓度 0 .5 % ,成型剂的pH值 6.4,菌体量 0 .5 g/g固定化细胞 ,固化时间 3 6h的条件下 。

A new method for immobilizing microbial cells with PVA carrageenin was presented and the better process conditions were obtained . The results showed that the better immobilization conditions were as follows: PVA 10%, carrageenin 0.5%, pH 6.4, microbial cells 0.5 g/g immobilized cells, immobilization time 36h.