细胞视黄酸结合蛋白Ⅱ、表皮型脂肪酸结合蛋白及Ki-67在乳腺浸润性导管癌中的表达及相关性

Expression of CRABPⅡ,E-FABP and Ki67 in Breast Invasive Ductal Carcinoma and heir Correlation

目的研究细胞视黄酸结合蛋白(CRABP)Ⅱ、表皮型脂肪酸结合蛋白(E-FABP)和Ki-67在乳腺浸润性导管癌中的表达情况及三者的相关性。方法采用免疫组织化学检测2001年1月-2007年12月手术切除的152例乳腺浸润性导管癌中CRABPⅡ、E-FABP和Ki-67的表达。结果在浸润性导管癌中,CRABPⅡ在Ki-67阴性组的阳性率高于Ki-67阳性组(P<0.05),相反地,E-FABP在Ki-67阳性组的阳性率高于Ki-67阴性组(P<0.05)。CRABPⅡ和Ki-67表达呈负相关(rS=0.432,P<0.05);E-FABP和Ki-67表达呈正相关(rS=0.842,P<0.05)。E-FABP和Ki-67的表达具有协同性,E-FABP和Ki-67共同表达与肿瘤的转移有关(P<0.05)。单因素生存分析显示,E-FABP的阳性表达患者、Ki-67的阳性表达患者以及E-FABP和Ki-67的共同阳性表达患者的预后差(P<0.05)。多因素生存分析提示E-FABP的表达(RR=4.223,P=0.012)和TNM分期(RR=8.412,P=0.000)是影响浸润性导管癌患者预后的独立危险因素。结论在乳腺浸润性导管癌中,CRABPⅡ和E-FABP与肿瘤细胞的增殖有关,CRABPⅡ抑制细胞增殖,E-FABP促进细胞增殖。E-FABP和Ki-67在浸润性导管癌的发生、发展中起协同作用,两者的阳性表达可能对评估肿瘤的转移和患者的预后有一定价值。

Objective To investigate the expression of CRABP Ⅱ , E-FABP and Ki67 in breast invasion ductal carcinoma （IDC） and their correlation. Methods CRABP Ⅱ , E-FABP and Ki67 were detected in 152 cases of IDC removed from January 2001 to December 2007 by immunohistochemical staining. Results The expression of CRABP Ⅱ had a recognizable advantage in Ki67-negative group than Ki67-positive group. E-FABP had a recognizable advantage in Ki67-positive group than Ki67-negative group （P 〈 0.05）. There was a significant negative correlation between CRABP H and Ki67 （r=-0.432, P 〈 0.05）, while positive correlation existed between E-F＇ABP and Ki67 （r=0.842, P 〈 0.05）. The expression of Ki67 coordinated with E-FABP, and their co-expression was correlated with tumor metastasis （P 〈 0.05）. Single survival analysis indicated that the E-FABP positive expression, Ki67 positive expression and their co-expression were associated with postoperative survival rate of IDC patients （P 〈 0.05）. The multivariate analysis using the COX regression model identified that E-FABP was independent prognostic factor of IDC patients （P 〈 0.0）. Condusions CRABP Ⅱ and E-FABP are relevant to cell proliferation in IDC patients. CRABP Ⅱ inhibits cell proliferation, while E-FABP promotes cell proliferation. The expression of E-FABP and Ki67 has a synergistic action in IDC, which is related to tumor metastasis and prognosis.