盐胁迫对绿豆下胚轴氧化还原态的影响

Effects of Salt Stress on Redox Status of the Hypocotyls of Vigna radiata L.

以150 mmol·L-1NaCl对绿豆下胚轴进行胁迫处理,测定其长度和鲜重以及GSH/GSSG、AsA/DHA、NADH/NAD+、NADPH/NADP+比值。结果表明,对照和处理48 h时上述指标分别相当于0 h时的493.1%与206.9%、661.0%与308.2%、118.1%与72.2%、444.4%与44.4%、96.6%与194.3%和97.1%与154.4%。可见,盐胁迫处理显著抑制绿豆下胚轴生长可能与其氧化还原态的显著改变有关。

To investigate the relationship between salt stress and redox status in the hypocotyls of Vigna radiata, the levels of length and fresh weight along with the ratios of reduced and oxidized glutathione （GSH/ GSSG）, ascorbic acid （AsA/DHA）, nicotinamide adenine dinucleotide （NADH/NAD＋） and nicotinamide adenine dinucleotide phosphate （NADPH/NADP＋） were determined in the three-old-day seedlings treated with 150 mmol＇L-1 NaC1 for 48 h. Above parameters of control and treatment at 48 h were equal to 493.1% vs 206.9%, 661.0% vs 308.2%, 118.1% vs 72.2%, 444.4% vs 44.4%, 96.6% vs 194.3% and 97.1% vs 154.4% of those at 0 h, respectively. The results suggested that the marked inhibition of growth in the hypocotyls of V. radiata by salt stress might be related to the significant variations of redox status.