摘要
本研究在1216名受检者中探讨维生素D结合蛋白基因rs2282679A/C多态性与维生素D水平及维生素D缺乏之间的关系。运用标准化问卷收集饮酒、吸烟、药物服用史等,并使用放射免疫比浊法测定血清25-羟维生素D[25(OH)D]。抽提DNA,以SNaPshot方法检测维生素D结合蛋白基因型。1216名受检者包括457名女性(37.6%),478名受检者(39.3%)为维生素D缺乏[25(OH)D〈50nmol/L],386名受检者(31.7%)为超重/肥胖。维生素D结合蛋白CC、AC、AA三个基因型的频率分别为8.7%、41.0%和50.3%;基因型和等位基因频率分布在维生素D正常者及维生素D缺乏者之间有显著差异(P〈0.05)。在调整协变量前后,CC、AC、AA三个基因型之间25(OH)D水平的差异均有统计学意义(P〈0.01)。与整个研究人群相比,AA型维生素D缺乏的患病风险低22%(OR=0.78,95% CI0.65~0.93,P=0.006)。和CC型相比,AA型维生素D缺乏的患病风险低36%(OR=0.64,95% CI0.42~0.98,P=0.006);AC型和CC型相比,维生素D缺乏患病风险差异无统计学意义(OR=0.87,95% CI0.57~1.34,P=0.53)。总之,维生素D结合蛋白rs2282679A/C多态性与血清25(OH)D水平和维生素D缺乏的患病风险显著相关。
To investigate the association of rs2282679 A/C polymorphism in vitamin D binding protein gene with vitamin D deficiency in 1 216 participants. A standardized questionnaire was applied to collect information on alcohol consumption, smoking habits, and use of medications. Serum 25-hydroxyvitamin D [25(OH)D] was determined by radioimmunoassay. Vitamin D binding protein genotypes were determined by SNaPshot method. 1 216 participants included 457 women(37.6%), 478(39.3%)with vitamin D deficiency [25(OH)D〈50 nmol/L], and 386(31.7%)overweight/obese participants. The frequencies of rs2282679 CC, AC, and AA genotypes were 8.7%, 41.0%, and 50.3%, respectively. The distributions of genotypes and alleles differed significantly between participants with sufficient vitamin D and those with vitamin D deficiency(P〈0.05). There were significant differences in the serum levels of 25(OH)D between three genotypes before and after being adjusted for covariates(P〈0.01). AA had a 22%(OR=0.78, 95% CI 0.65-0.93, P=0.006)lowered risk of vitamin D deficiency compared with the whole studied population. Compared with CC genotype, AA had a 36% lowered risk of vitamin D deficiency(OR=0.64, 95% CI 0.42-0.98, P=0.006), while there was no significant difference between AC and CC genotypes(OR=0.87, 95% CI 0.57-1.34, P=0.53). In conclusion, the vitamin D binding protein rs2282679 A/C polymorphism was significantly associated with serum level of 25(OH)D and risk of vitamin D deficiency.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2013年第10期846-848,共3页
Chinese Journal of Endocrinology and Metabolism
基金
国家自然科学基金项目(30960137)