代谢综合征合并结直肠癌患者脂肪组织IGF-Ⅰ、ERK、GLUT4 mRNA表达水平的变化及临床意义

The significance of genetic expression of IGF-Ⅰ, ERK, and GLUT4 in adipose tissue of patients with metabolic syndrome and colorectal cancer and its significance

分析代谢综合征合并结直肠癌患者大网膜脂肪组织中胰岛素样生长因子Ⅰ（IGF-Ⅰ）、细胞外信号调节激酶（ERK）和葡萄糖转运蛋白4（GLUT4）mRNA表达水平。采用RT-PCR技术检测患者大网膜脂肪组织中IGF-Ⅰ、ERK和GLUT4的mRNA表达水平。结果显示：（1）代谢综合征组大网膜脂肪组织IGF-Ⅰ和ERK的mRNA表达显著高于对照组（P〈0.01），其中结直肠癌亚组高于非肿瘤亚组（P〈0.01）。GLUT4的mRNA表达显著低于对照组（P〈0.01）。（2）ERK与IGF-Ⅰ的mRNA表达呈显著正相关（r=0.608,P〈0.01）。血清空腹胰岛素与ERK、IGF-Ⅰ的mRNA表达呈显著正相关（r=0.538、0.439,P〈0.01），与GLUT4呈显著负相关（r=-0.457,P〈0.01）。IGF-Ⅰ和ERK联合与代谢综合征患者并发结直肠癌有关，GLUT4在代谢综合征中的表达下调可能与代谢综合征脂肪组织发生胰岛素抵抗有关。

To explore the mRNA expression of insulin-like growth factor-Ⅰ（IGF-Ⅰ）, extracellular signal regulated kinase（ERK）, and glucose transporter 4（GLUT4）in greater omental adipose tissue of patients with metabolic syndrome and colorectal cancer. The mRNA expression of IGF-Ⅰ, ERK, and GLUT4 in greater omental adipose tissue of the subjects was measured by RT-PCR.（1）The mRNA expression level of IGF-Ⅰ and ERK in the metabolic syndrome group was significantly higher than that in the control group（P〈0.01）, while in colorectal cancer subgroup the expression was significantly higher than that in the non-colorectal cancer subgroup（P〈0.01）.The expression of GLUT4 was obviously lowered（P〈0.01）.（2）The expression of ERK was positively correlated with that of IGF-Ⅰ（r= 0.608, P〈0.01）.The fasting insulin was positively correlated with the expression of ERK and IGF-Ⅰ（r =0.538, 0.439, P〈0.01）, and negatively with that of GLUT4（r=-0.457,P〈0.01）. There may be relationship between ERK plus IGF-Ⅰ and metabolic syndrome complicated with colorectal cancer. The lowered GLUT4 expression may be related to insulin resistance in metabolic syndrome.