低氧预处理对大鼠海马神经元缺氧耐受性和IL-1β表达的影响

EFFECTS OF HYPOXIC PRECONDITIONING ON ANOXIC TOLERANCE AND IL 1β EXPRESSION IN CULTURED RAT HIPPOCAMPAL NEURONS

目的 :观察低氧预处理对大鼠海马神经元缺氧耐受性和白细胞介素 1β(IL 1β)表达的影响。 方法 :取培养 12d的两组 (对照组和低氧预处理组 )培养神经元 ,同时置于缺氧环境 (0 .9L/LN2 ,0 .1L/LCO2 )中培养 2、4、8和 12h。分别观察它们的形态变化和神经元存活数 ,并用抗rhIL 1β单克隆抗体进行免疫组织化学染色 ,观察缺氧对大鼠海马培养神经元IL 1β表达的影响。 结果 :经低氧预处理的海马神经元缺氧后IL 1β表达较对照组减弱 ,神经元损伤程度减轻 ,神经元存活数明显高于对照组。结论 :低氧预处理可使海马培养神经元对缺氧产生耐受 ,其中IL

Aim: To study the effects of hypoxic preconditioning on anoxia tolerance and IL 1β expression in cultured rat hippocampal neurons after anoxia. Methods: 12 day cultured hippocampal neurons in control and hypoxic preconditioning group were exposed to ano xic environment(0.9 L/LN 2+0.1 L/LCO 2) for 2,4,8 and 12 h. The neurons were immunocytochemically stained using the antiserum against rhIL 1β,The number of survival neurons and the mean optical density (OD) of IL 1β expression were investigated. Resutls: The morphological changes and IL 1β expression induced by acute anoxia in hypoxic preconditioning neurons were significantly less than those in control. The number of survival neurons was higher in the hypoxia preconditioning group than that in control group after acute hypoxia. Conclusion: Hypoxia preconditioning can induce the development of anoxia tolerance in cultured hippocampal neurons. The decreased IL 1β in hippocampal neurons may be an adaptive reaction to acute anoxia.