兴奋性氨基酸加强高原低氧大鼠下丘脑前生长抑素原mRNA的表达

EXCITATORY AMINO ACID ENHANCE PREPRO SOMATOSTATIN mRNA EXPRESSION INDUCED BY ALTITUDE HYPOXIA IN THE RAT HYPOTHALAMUS

目的和方法 :应用大鼠高原低氧模型及原位杂交技术和氨基酸测定法 ,研究下丘脑前生长抑素原 (PPS)mRNA表达和谷氨酸 (Glu)、天门冬氨酸 (Asp)含量的变化。 结果 :高原低氧组大鼠下丘脑Glu和Asp的含量明显增多 ,室周核、室旁核、弓状核PPS mRNA阳性神经元数目显著增加 ;而NMDA受体拮抗剂氯铵酮 ,虽然对Glu和Asp含量无明显影响 ,但可使高原低氧大鼠下丘脑PPS mRNA阳性神经元数目减少。结论 :下丘脑生长抑素可能参与了高原低氧反应 ,Glu通过NMDA受体可增强高原低氧大鼠下丘脑PPS

Aim and Methods:Contents of glutamate (Glu),asparate (Asp) and expression of prepro somatostatin mRNA (PPS mRNA) in rat hypothalamus were measured by using imitated altitude hypoxia rat model, amino acid analyzer and in situ hybridization technique.Results: After rats were subjected to altitude hypoxia,contents of Glu and Asp in hypothalamus and PPS mRNA expression in periventricular nucleus (PeVN),paraventricular nucleus (PaVN) and arcuate nucleus (ArcN) were increased significiantly.Ketamine, a NMDA receptor antagonist,could decrease the number of PPS mRNA neurons in rat hypothalamus evoked by altitude hypoxia, but had no effect on Glu and Asp contents evoled by altitude hypoxia.Conclusion:It is suggested that somatostatin maybe paticipate in altitude hypoxia reaction,Glu can enhance PPS mRNA expression through NMDA receptor.