不同应激损伤所致血管内皮细胞中vWF的变化

THE CHANGE OF vWF IN VASCULAR ENDOTHELIAL CELLS UNDER DIFFERENT STRESS

目的 :通过检测血管内皮细胞 (VEC)中vWF的变化 ,评价VEC的损伤。方法 :利用免疫组化技术对体外培养的猪肺动脉内皮细胞 (PAEC)和大鼠主动脉内皮细胞 (AEC)中vWF进行免疫荧光标记 ,通过流式细胞仪对vWF的细胞阳性率和荧光强度进行定性、定量分析 ,观察低氧和低温所引起的vWF变化。结果 :正常猪PAEC和大鼠AEC的vWF阳性率在 80 %左右 ,阳性程度较高。但缺氧或冷冻损伤可使PAEC和AEC的vWF阳性率不同程度的降低 ,同时 ,阳性细胞功能代偿 ,合成vWF增加 ,阳性细胞的荧光强度增加。结论

Aim: To correlate the injury of vascular endothelial cells during various pathological conditions with the change of vWF (von Willebrand Factor) in different VEC lines. Methods: Flow cytometer(FCM) were used to defect the immunoflourescent stained vWF in pulmonary artery endothelial cells (PAEC) of pig and aortic endothelial cells(AEC) of rats. Results: The positive rates of vWF in PAEC of pigs is similar with that in AEC of rats under normal condition, but it decreased differently after hypoxic or cold injury. It was very interesting that the mean fluorescence intensity of positive PAEC or AEC exposed to hypoxia or cold elevated significantly compared with those of control. Conclusions: The change of vWF in VEC can be used to evaluate the function of VEC under different stress.