乏氧诱导因子在牙周炎牙龈中的表达研究

Expression of hypoxia-inducible factor in gingival tissues with periodontitis

目的:比较观察乏氧诱导因子(HIF)在正常和牙周炎的牙龈组织中的表达差异,探讨HIF在牙周炎发生发展过程中的可能作用。方法:采用免疫组织化学(PV两步法)染色,分别检测20例健康成人及20例重度牙周炎患者(PD≥6 mm)牙龈组织中HIF-1α蛋白的表达;并采用Image-pro plus 6.0图像分析系统,半定量分析比较两组表达水平的差异。结果:HIF-1α在正常对照组牙龈组织中无明显阳性表达;在牙周炎组牙龈组织的上皮全层及结缔组织中均呈阳性表达,两组差异具有统计学意义(P<0.05)。结论:HIF-1α参与牙周炎牙龈组织的病理改变过程,可能在牙周炎的发生发展及修复机制中具有重要意义。

AIM：To investigate the expression of hypoxia-inducible factor （HIF） in normal and inflammatory gingival tissues.METHODS：Gingival tissues were obtained from 20 healthy volunteers and 20 patients with periodontitis.HIF-1 α protein expression was detected with Power VisionTM two-step immunohistochemical method.Image -pro plus 6.0 was used to semi-quantify the HIF expression.RESULTS：HIF expression was negligible in healthy gingival tissues,while it was strongly expressed in gingival tissues of periodontitis patients.Significant statistical difference was found between the two groups （P ＜ 0.05）.CONCLUSION：HIF might play an important role in periodontitis initiation and progression and participate in the periodontal tissue remodeling.