子宫内膜细胞诱导胚胎干细胞修复小鼠受损子宫内膜

Endometrial Cells Inducing Embryonic Stem Cells to Repair the Injured Mouse Endometrium

目的：探讨小鼠胚胎干细胞（ESCs）经体外与子宫内膜细胞共培养后移植于内膜损伤小鼠的子宫腔是否有助于损伤的修复并降低其成瘤性。方法：制备新鲜子宫内膜损伤的小鼠模型，利用带EGFP基因标记的小鼠胚胎干细胞（ESC-EGFP）与同种子宫内膜共培养后移植至模型鼠宫腔；观察ESC-EGFP在模型鼠宫腔内滞留情况、对损伤内膜的修复影响以及对干细胞移植后成瘤性的影响。结果：将与子宫内膜共培养后的ESC-EGFP移植到新鲜损伤的模型鼠宫腔内，可使损伤子宫的外形较PBS对照组保持更好，移植后1-4周的损伤子宫大体标本均可见荧光；组织病理可见新生子宫内膜腺体样组织，并显示GFP免疫组织化学标记；且在有限观察时间内无肿瘤形成。结论：ESCs与子宫内膜细胞共培养方式可能诱导ESCs分化的方向，而在体子宫腔损伤的局部环境有利于胚胎干细胞定植与进一步向子宫内膜分化。

Objective： To investigate whether the embryonic stem cells （ESCs） co-cultured with endometrial cells could help the endometrium reparation and reduce the neoplasia of ESCs. Methods： The mouse model with freshly injured endometrium was established. ESCs derived from EGFP transgenic mouse （ESC-EGFP） were transplanted to endometrium-injured mouse after cultured with endometrial cells. The retention and the effects of ESCs on the reparation of injured endometrium and the tumorigenicity of post-transplanted stem cells were observed. Results： The co-cultured ESC-E6FP could survive in the freshly endometrium-injured mouse＇s uterine cavities and keep the injured uterus in better shape compared with PBS group. The fluorescent light could be observed on the injured uterus in the first 4 weeks after the transplantation, with the new generated endometrial gland-like tissue observed by histopathology and GFP immunohistochemical staining positive. The evidence of endometrium neoplasia was not found during the limited observing period. Conclusion： Co-culture with endome- trial cells may induce the differentiation of ESCs. And the environment of injured uterus cavity may avail to the implantation and differentiation of the ESC.