VEGF基因多态性与广西壮族妇女复发性流产的相关性研究

Association of VEGF Single Nucleotide Polymorphisms and Patients with Recurrent Spontaneous Abortion in Guangxi Zhuang Women

目的:探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)-2578C/A、-1154G/A、-634G/C、-460C/T和+936C/T位点单核甘酸多态性(single nucleotide polymorphisms,SNPs)与广西壮族妇女复发性流产(RSA)发病的关联。方法:应用PCR-限制性片段长度多态性技术(PCR-RFLP)检测RSA组(120例,病例组)和健康对照组(110例)的VEGF 5个SNPs位点基因型频率分布情况。结果:VEGF-2578C/A、-1154G/A、-634G/C、-460C/T和+936C/T的SNPs位点基因型和等位基因频率分布在病例组和对照组间均无统计学差异(P>0.05)。结论:VEGF-2578C/A、-1154G/A、-634G/C、-460C/T和+936C/T位点的基因多态性可能与广西壮族妇女RSA发病风险不相关。

Objective： To investigate the association of single nucleotide polymorphisms （SNPs） in vascular endothelial growth factor （VEGF） gene with the risk of recurrent spontaneous abortion （RSA） in Guangxi Zhuang women. Methods： Totally 120 patients suffering RSA and 110 controls were enrolled in this study. Genotypes of VEGF were measured by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） method. Results： No significant difference was observed in allele and genotype distributions of the VEGF -2578C/A, -1154G/ A, -634G/C, -460C/T and ＋936C/T polymorphisms between patients and controls （P〉0.05）. Conclusion： The SNP of VEGF -2578C/A, -1154G/A, -634G/C, -460C/T and ＋936C/T may have no association with RSA in Guangxi Zhuang women.