冻融卵裂期胚胎单个卵裂球体外发育潜能的初步探讨

Preliminary Investigation on the in vitro Developmental Potential of Single Blastomeres Derived from Frozen-thawed Cleavage-stage Embryos

目的：探讨体外受精．胚胎移植0VF—ET）治疗周期中冻融胚胎来源的单卵裂球体外发育潜能。方法：20例IVF-ET术生育成功后捐赠的91枚多余冷冻的胚胎，解冻后分为〈6-细胞组、6～8-细胞组和〉8-细胞组，将每枚胚胎中的卵裂球分离、取出，每个单卵裂球单独培养于一个液滴中，每24h观察并比较各组间单卵裂球发育情况的差异。结果：6～8-细胞组单卵裂球的卵裂率、紧密化率和囊胚形成率显著高于其它各组。结论：单卵裂球体外培养发育能力及时序与其来源的胚胎发育潜力及发育阶段密切相关；辅助生殖FET周期应优先选择6～8-细胞的胚胎进行移植。

Objective： To investigate the developmental potentiality of single isolated blastomeres of human frozen-thawed embryos in vitro during in vitro fertilization and embryo transfer （IVF-ET）. Methods： Ninty-one frozen embryos from 20 patients who accepted IVF-ET and were succesfully pregnant were divided into three groups： 〈6-cell, 6-8-cell and 〉8-cell. Single isolated blastomere was separated with the technology of biopsy and continued culturing in one single culture drop in vitro. During this period, the development characteristics of blastomeres were observed every 24 h. Results： The rates of cleavage, compaction and blastocyst of single isolated blastomere from the 6-8-cell group were significantly higher than those in other two groups. Conclusion： The developmental capacity and timing of single isolated blastomere were closely related to developmental potential and developmental stage of its embryos. Embryos of 6-8-cell should be a priority for the frozen-thawed embryo transfer frET） cycle of assisted reproduction technology （ART）.