胚胎培养体系研究进展

Advances in Embryo Culture System

以往人们对体外胚胎发育的研究大多集中在培养液中的化学成分上,而培养体系中的物理微环境对胚胎发育的影响往往易被忽略。近年来,胚胎培养体系已从静置的微滴、微穴培养逐渐发展到动态的微流体培养。静置培养体系因培养效果稳定,且易操作,一直占据主导地位。动态培养体系不但能为胚胎补充新鲜的培养液,并还能及时清除胚胎产生的代谢产物,但其操作更复杂,限制了其在临床的广泛应用。本文就各种培养体系在体外胚胎培养中的研究进展综述如下。

Over the last two decades, the immense amount of data aiming at improving embryo development in vitro has involved the chemical composition of the culture media, however, physical requirements for embryos development in vitro have been largely neglected. Recently, the culture system has been developed from simple static microdrop and microwell culture to dynamic microfluid culture. Static embryo culture has been the predominate method employed to date for its stable culture effect and easy operation. Dynamic embryo culture system will not only be able to add fresh culture medium, and also the timely removal of metabolites produced by the embryo. As theoretically promising as dynamic culture appears, one of the limitations of these systems is their relative complexity in comparison to static culture devices. The current progress in embryo culture platforms on the development of embryo would be re- viewed in this article.