摘要
以鹤顶兰原球茎为材料,使用秋水仙素进行了四倍体的离体诱导。结果表明,采用秋水仙素浸泡法可以诱导鹤顶兰原球茎发生倍性变化,含0.02%秋水仙素的"花宝一号"溶液浸泡处理6 d诱导率最高,达22.5%。细胞学鉴定发现,诱变成活植株中有3株为四倍体,其植株形态、气孔大小、气孔密度与二倍体存在差异,细胞核明显增大。
Tetraploids ofPhaius tankervilleae were induced through the treatment ofprotocorms with colchicine in vitro. The results showed that ploidy levels of protocorms changed with colchicine soaking and the highest induction rate of 22.5% was obtained by immersing protocorms into 0.02% colchicine solution for 6 days. Cytological determination of regenerated plants indicated that three tetraploid plantlets were obtained among mutated plants survived, and the morphology, the number and size of stomata of the mutants were different from those of the diploid plants, with obviously bigger nucleus.
出处
《园艺学报》
CAS
CSCD
北大核心
2013年第10期2033-2038,共6页
Acta Horticulturae Sinica
基金
教育部热带作物新品种选育工程中心与作物学重点学科联合资助项目(lhxm-2012-7)
海南省自然科学基金项目(313032)
公益性行业(农业)科研专项经费项目(201203071)
关键词
鹤顶兰
秋水仙素
四倍体
原球茎
Phaius tankervilleae
colchicine
tetraploid
protocorm
