摘要
丁二酸是微生物三羧酸循环中重要的代谢中间产物,广泛用于生物高分子、食品与医药等行业,市场潜在需求量巨大。文中从3个方面归纳了国内外生物基丁二酸研究进展:能够过量积累丁二酸的微生物的发现和筛选,产丁二酸工程菌构建中所采用的基因工程策略及代谢工程技术,丁二酸发酵过程控制与优化。最后,讨论了微生物法生产丁二酸今后的研究方向。
Succinic acid is one of the key intermediates in the tricarboxylic acid cycle (TCA)and has huge potentials in biopolymer, food, medicine applications. This article reviews recent research progress in the production of succinic acid by microbial fermentation, including discovery and screening of the succinic-acid-producing microbes, the progress of genetic engineering strategy and metabolic engineering technology for construction of succinic acid-producing strains, and fermentation process control and optimization. Finally, we discussed the limitation of current progress and proposed the future research needs for microbial production of succinic acid.
出处
《生物工程学报》
CAS
CSCD
北大核心
2013年第10期1386-1397,共12页
Chinese Journal of Biotechnology
基金
国家自然科学基金(No.21076105)
国家重点基础研究发展计划(973计划)(No.2009CB724701)
江苏高校优势学科建设工程项目资助~~
关键词
丁二酸
菌种选育
基因工程
代谢工程
过程强化
succinic acid
strain breeding
genetic engineering
metabolic engineering
process control and optimization