人羊膜间充质干细胞的超微结构观察

Ultrastructure of human amniotic mesenchymal stem cells

目的观察羊膜间充质干细胞的超微结构。方法分离和培养人羊膜间充质干细胞(AMSC),进行传代培养、扩增,在显微镜下观察细胞形态。对传代培养至第3代的hAMSC进行流式检测和鉴定细胞表型,并在透射和扫描电子显微镜下进行超微结构的观察。结果人AMSC呈梭形和多角形;高表达表面标志CD44,CD90,CD105和波形蛋白,不表达CD34和CD45。扫描电镜下可见细胞表面有大量小泡和微绒毛突起;透射电镜下见到胞核较大,呈圆形或椭圆形;胞质内线粒体、粗面内质网和高尔基体等细胞器数量丰富,有较多小泡。结论从羊膜中分离培养的细胞具有间充质干细胞的生物学特性的超微结构。丰富的粗面内质网和高尔基体,提示,AMSC具有产生大量分泌蛋白的功能,可能是羊膜间充质干细胞旁分泌作用的基础。

Objective To observe the ultrastructure of human amniotic mesenchymal stem cells（AMSCs）. Meth- ods Human AMSC from full-term newborn amnion were isolated and cultured followed by and then subculture and amplification. Cell morphology was observed by microscopy. The Flow detection and identification of human AMSCs phenotype of at passage 3 were analyzed. Transmission and scanning electron microscopy were used to ob- serve the ultrastructure of human AMSC. Results The appearance of human AMSC was spindle-shaped and po- lygonal, the hAMSC expressed immunophenotype CD44, CDg0, CD105 but not CD34, CD45. The short and thick microvilli processes were seen at the surface of human AMSC by scanning electron microscope, morpholigies of human AMSC were seen under transmissionelectron microscope, the cell was in a relatively active period in which a large and round or oval nucleus in the one cell were observed, The chromatin sparsely distributed in the nucle- us, and concentrated in cytoplasm. The organelles of mitochondria, rough endoplasmic reticulum, golgi apparatusand small vesicle were cells has the biological. paracrine function. rich in chromation. The results suggest Conclusions Ultrastructure of human amniotic that it secretory protein function, which is likely to mesenchymal stem be the basis of cellparacrine function.