Wnt5a及其受体Fzd2在肌萎缩脊髓侧索硬化症转基因鼠内嗅皮层中的表达变化

Expression of Wnt5a and its receptor Fzd2 in the entorhinal cortex of amyotrophic lateral sclerosis transgenic mice

探讨Wnt5a及其受体FZd2在95、108d和122d肌萎缩脊髓侧索硬化症（ALs）转基因鼠大脑内嗅皮层中的表达情况。方法：取95、108d和122dALS转基因鼠大脑皮质，应用免疫荧光和RT-PCR技术，检测Wnt5a和Fzd2在大脑内嗅皮层中的表达变化。结果：免疫荧光结果显示，与同窝野生型鼠比较，95、108d和122d龄ALS转基因鼠大脑内嗅皮层中Wnt5a阳性细胞明显增多。免疫荧光双标结果显示，Wnt5a标记的阳性细胞与GFAP和β-tubulinIII共表达，ALS转基因鼠Wnt5a／GFAP双阳性细胞增多。与同窝野生型鼠比较，ALS转基因鼠Wnt5amRNA水平在95、108d和122d均升高。Wnt5a受体Fzd2表达情况与Wnt5a一致。结论：WntSa和Fzd2在ALS转基因鼠内嗅皮层中表达增多，表明Wnt5a及其受体Fzd2升高与肌萎缩脊髓侧索硬化症密切相关。

Objective： To observe the expression changes of Wnt5a and its receptor Fzd2 in the entorhinal cortex of adult amyotrophic lateral sclerosis （ALS） transgenic mice. Methods： The expressions of Wnt5a and Fzd2 in the entorhinal cortex of ALS transgenic mice at the age of 95 d, 108 d and 122 d were detected with immunohistochemistry technique and RT-PCR. Results： Immunofluorescence results showed that WntSa positive cells were increased significantly in the entorhinal cortex of ALS transgenic mice at different ages, compared with wild-type mice. Double-labeling results showed that Wnt5a-labeled cells were co-expressed with GFAP and β-tuhulin III in the ALS transgenic mice and wild-type mice, and WntSa/GFAP double positive cells were increased in ALS mice. Compared with the wild-type mice, Wnt5a mRNA was increased at 95 d, 108 d and 122 d. The expression of Fzd2 was similar to that of WntSa. Conclusion： The expressions of WntSa and Fzd2 are increased in the entorhinal cortex of ALS transgenic mice, which may be associated with the pathogenesis of AIRS.