巢蛋白在成年小鼠室周器官细胞的表达及其意义

Expression of nestin protein in circumventricular organs in adult mice and its significance

目的：检测成年小鼠巢蛋白在室周器官细胞的表达及其意义。方法：成年BALB／c雄性小鼠，免疫组织化学检测巢蛋白在室周器官细胞的表达。结果：巢蛋白在最后区、正中隆起、穹隆下器、连合下器和脉络丛细胞均有表达。大部分巢蛋白阳性表达细胞有长短不一的胞突。结论：在成年小鼠室周器官分布有大量的巢蛋白阳性表达细胞，表明成年小鼠室周器官可能存在具有增殖和分化潜能的神经干细胞／祖细胞。

Objective： To observe the expression of nestin positve cells in the adult mouse circumventricular organs （CVOs） and subventricular zone and discuss its significance. Methods： CVOs of adult mice （aged 7-8 weeks, male） after conventional heart perfusion were detected by immunohistochemical staining for nestin positive cells. Results： The nestin positive cells were found in area postrema, median eminence, subfornical organ, subcommissural organ, and choroid plexus. A majority of nestin positively expressing cells had a long or short process. Conclusion： Abundent nestin positively expressing cells were found in CVOs of adult mice, suggesting the existence of the neural stem/progenitor cells, which can proliferate and differentiate into new neurons and/or glia cells. A part of nestin positively expressing cells may be nestin expressing neurons or glia cells.