一氧化氮供体增强大鼠记忆及其与脑内ADP-核糖基转移酶的关系

Relationship between memory enhancement induced by nitric oxide donor and brain ADP-ribosyltransferase in rats

为探讨与学习记忆有关的一氧化氮 (nitricoxide ,NO)信号转导通路 ,本文用NO供体硝普钠 (sodiumnitroprusside,SNP)或同时给予ADP 核糖基转移酶 (ADP ribosyltransferase ,ADPRT)抑制剂尼克酰胺 (nicotinamide ,NIC)侧脑室内注射 ,观察其对大鼠学习记忆行为的影响。并用高效液相色谱法测定脑内ADPRT活性。结果表明 ,SNP (0 36 μgicv)使大鼠在被动回避反应的短时与长时记忆和在主动回避反应的学习记忆能力增强。首次发现ADPRT抑制剂NIC (1 5mgicv)阻断SNP增强记忆的效应。还首次报告了学习训练或加上SNP (icv)使大鼠海马和大脑皮层的ADPRT活性显著升高。本文结果支持ADPRT可能是NO作用的一种靶分子的观点。

In order to explore the signal transduction pathway of nitric oxide (NO) related to learning and memory, the effects of intracerebral ventricular injection (icv) of NO donor sodium nitroprusside (SNP) alone or along with ADP ribosyltransferase (ADPRT) inhibitor nicotinamide (NIC) on learning and memory behavior were investigated in rats. Brain ADPRT activity was analyzed using high performance liquid chromato^graphy. The results showed that SNP (0 36 μg, icv) facilitated short term and long term memory in passive avoidance response and elevated learning and memory ability in active avoidance response. NIC (1 5 mg, icv) blocked the effect of SNP. Training in active avoidance task raised ADPRT activity in hippocampus. Treatment with SNP (icv) before training caused significant elevation of ADPRT activity in hippocampus and cerebral cortex. The results above support the view that ADPRT may be one of the target molecules of NO action. [WT5HZ]