口腔扁平苔藓中乳腺珠蛋白表达变化的初步研究

Expression of secretoglobin family 2A member 2 in oral lichen planus lesions

目的 从转录、蛋白水平验证口腔扁平苔藓（oral lichen planus,OLP）病损差异表达基因乳腺珠蛋白（secretoglobin family 2A member 2,SCGB2 A2）的变化,探讨SCGB2A2在OLP发病中的作用.方法 分别应用实时荧光定量反转录PCR、蛋白质印迹法及免疫组织化学等方法检测16例OLP病损（OLP组）和10例正常对照（正常对照组）中SCGB2A2转录水平及蛋白表达情况,采用单因素方差分析对组间结果进行比较,以双侧P＜0.05为差异有统计学意义.结果 OLP组SCGB2A2蛋白水平（1.02±0.11）显著高于正常对照组（0.48 ±0.09）,两组差异有统计学意义（P=0.002）;OLP组SCGB2A2的转录水平[M（Q1,Q3）][1.68（1.60,1.83）]则显著低于正常对照组[6.32 （5.24,10.87）],两组差异有统计学意义（P＜0.05）.结论 SCGB2A2参与了OLP的发病,其作用机制尚有待进一步研究。

Objective To examine the expression of secretoglobin family 2A member 2 （SCGB2A2） （mammaglobin A,MGB1） in oral lichen planus （OLP） lesions.Methods Sixteen OLP patients and 10 healthy controls were included in this study.The real time reverse transcription-PCR （RT-PCR）,Western blotting and immunohistochemistry（IHC） were used to determine the mRNA and protein of SCGB2A2.Results Compared with healthy controls （0.48 ± 0.09）,the expression of SCGB2A2 protein in OLP lesions significantly increased （1.02 ± 0.11） （P 〈 0.05）.However,the mRNA level of SCGB2A2 in lesions was significantly lower than that in controls （P 〈 0.05）.Conclusions These results suggest that SCGB2A2 may be involved in pathogenesis of OLP.