摘要
目的 从转录、蛋白水平验证口腔扁平苔藓(oral lichen planus,OLP)病损差异表达基因乳腺珠蛋白(secretoglobin family 2A member 2,SCGB2 A2)的变化,探讨SCGB2A2在OLP发病中的作用.方法 分别应用实时荧光定量反转录PCR、蛋白质印迹法及免疫组织化学等方法检测16例OLP病损(OLP组)和10例正常对照(正常对照组)中SCGB2A2转录水平及蛋白表达情况,采用单因素方差分析对组间结果进行比较,以双侧P<0.05为差异有统计学意义.结果 OLP组SCGB2A2蛋白水平(1.02±0.11)显著高于正常对照组(0.48 ±0.09),两组差异有统计学意义(P=0.002);OLP组SCGB2A2的转录水平[M(Q1,Q3)][1.68(1.60,1.83)]则显著低于正常对照组[6.32 (5.24,10.87)],两组差异有统计学意义(P<0.05).结论 SCGB2A2参与了OLP的发病,其作用机制尚有待进一步研究。
Objective To examine the expression of secretoglobin family 2A member 2 (SCGB2A2) (mammaglobin A,MGB1) in oral lichen planus (OLP) lesions.Methods Sixteen OLP patients and 10 healthy controls were included in this study.The real time reverse transcription-PCR (RT-PCR),Western blotting and immunohistochemistry(IHC) were used to determine the mRNA and protein of SCGB2A2.Results Compared with healthy controls (0.48 ± 0.09),the expression of SCGB2A2 protein in OLP lesions significantly increased (1.02 ± 0.11) (P 〈 0.05).However,the mRNA level of SCGB2A2 in lesions was significantly lower than that in controls (P 〈 0.05).Conclusions These results suggest that SCGB2A2 may be involved in pathogenesis of OLP.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2013年第10期606-609,共4页
Chinese Journal of Stomatology
基金
国家自然科学基金(81000446)
教育部高等学校博士学科点专项科研基金(20100171120105)
广东省自然科学基金(10451008901005050)
关键词
扁平苔癣
口腔
基因表达
乳腺珠蛋白
Lichen planus,oral
Gene expression
Secretoglobin family 2A member 2