土曲霉(Aspergillus terreus)产生洛伐他汀(lovastatin)的研究

STUDY ON PRODUCTION OF LOVASTATIN BY Aspergillus terreus

从土曲霉Ａｓｐｅｒｇｉｌｌｕｓ ｔｅｒｒｅｕｓ ＣＡ９５１发酵液中，分离出一种白色针状晶体，经质谱、紫外光谱、红外光 谱和核磁共振谱分析，与文献报道的洛伐他汀（ｌｏｖａｓｔａｔｉｎ）基本一致。 Ａ．ｔｅｒｒｅｕｓ ＣＡ９５１经亚硝基胍诱变，选出 编号为ＣＡＮ １８７的突变株，其产生洛伐他汀的能力比ＣＡ９５１高６６％。摇瓶发酵实验研究了不同碳源、氮源和 水质对 ＣＡＮ１８７ 菌株产生洛伐他汀的影响，并进行了 ５００Ｌ发酵罐实验，发酵单位达９００ｍｇ／Ｌ左右。

A white crystal was isolated from fermentation broth of Aspergillus terreus CA951. Based on spectral data of mass, UV, IR and NMR (H and C), this compound was proved identical to lovastatin reported in references, a cholesterol- lowering agent. A high-producing strain named CAN187 was attained from hundreds of mutant after treated by NTG.It gave 1.66 times the yield of the original strain. Shaking flask fermentation studies were done to observe the effects of different carbon, nitrogen sources, and quality of water to lovastatin production. Experiment in 500L fermentor was also done, with lovastatin yield of about 900mg/L.