原生质体融合选育肌苷产生菌产氨短杆菌GMA-2802

SCREENING OF AN INOSINE-PRODUCING Brevibacterium ammoniagenes STRAIN GMA-2802 BY PROTOPLAST FUSION

以肌苷产生菌产氨短杆菌ＧＭＡ－２７２２（Ａｄｅ－、 Ｇｕａ－、ＶＢ１－、Ｒｉｆｒ）和 ＧＭＡ－２７７６（Ａｄｅ－、 Ｂｉｏｔｉｎ－、ＶＢ１－、Ｓｍｒ）为出发菌株，经原生质体融合，将目的标志加以组合，获得遗传性状稳定、摇瓶发酵６１ｈ，产肌苷２５．４ｇ／Ｌ的融合子 ＧＭＡ－２８０２（Ａｄｅ－、Ｇｕａ－、Ｂｉｏｔｉｎ－、ＶＢ１－、 Ｒｉｆｒ、Ｓｍｒ）。

An inosine-prefacing Brevibacterium ammoniagenes strain GMA 2802 that accumulated 25.4g/L after 6 hours in shaking flask was isolated from strain GMA-2722 (Ade-, Gua-, VB1-, Rifr) and strain GWA-2776 (Ade-, Biotin-, VB1-, Smr) by protoplast fusion. The genetic character of GMA-2802 (Ade-, Gua-, Biotin-, VB1-, Rifr,Smr) was stable.