TLR-5在溃疡性结肠炎中的表达及相关性研究被引量：1

Expression of TLR-5 in patients with ulcerative colitis

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摘要目的探讨TLR-5在溃疡性结肠炎发病中的作用。方法收集50例溃疡性结肠炎患者肠黏膜标本,通过RT-PCR检测肠黏膜中TLR-5 mRNA的表达量,ELISA检测NF-κB转录活性。另外收集30名健康志愿者作为对照组。结果中度及重度溃疡性结肠炎患者TLR-5 mRNA的表达量明显高于对照组(1.22±0.32、2.15±0.15、0.6±0.12,P<0.05);溃疡性结肠炎患者肠黏膜中NF-κB转录活性明显增强,与对照组比较差异有统计学意义(P<0.05);TLR-5 mRNA的表达量与溃疡性结肠炎的严重程度及炎症指标(ESR和CRP)呈正相关。结论 TLR-5可能通过活化NF-κB参与了溃疡性结肠炎的发病,且TLR-5可反映溃疡性结肠炎的疾病严重程度。 Objective To investigate the role of TLR-5 in the pathogenesis of ulcerative colitis （UC）. Methods 50 patients with UC and 30 heahhy volunteers were collected and analyzed. The level of TLR-5 was detected by RT- PCR, and activation of transcription of NF-KB was detected by ELISA. Results TLR-5 gene expression was significant- ly increased in UC patients with moderate and high disease activity compared with normal controls（ 1.22 ± 0.32, 2.15 ± 0.15, 0.6 ±0.12, P 〈0.05）. Compared with control group, activation of transcription of NF-KB in patients with UC was significantly increased （P 〈 0.05）. The level of TLR-5 was positively correlated with severity of disease and inflam- mation markers （ESR and CRP）. Conclusion TLR-5 induced activation of transcription factor NF-KB is involved in the pathogenesis of UC, and may reflect the severity of disease.

作者马小芬黄重发朱清裴小红卜全慧周怿

机构地区南京中医药大学中国人民解放军第

出处《胃肠病学和肝病学杂志》 CAS 2013年第10期990-992,共3页 Chinese Journal of Gastroenterology and Hepatology

关键词 TLR-5 NF—KB 溃疡性结肠炎严重程度炎症信号通路 TLR-5 NF-KB Ulcerative colitis Severity of disease Inflammation Signaling pathway

分类号 R574.62 [医药卫生—消化系统]

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