胆碱对鸡胚绒毛尿囊膜血管新生的促进作用

Choline promotes angiogenesis in chick embryo chorioallantoic membrane

目的:研究胆碱对鸡胚绒毛尿囊膜(CAM)血管新生的促进作用。方法:制备CAM模型,将氯化胆碱、人血管内皮细胞生长因子(hVEGF)和生理盐水分别通过载体加到CAM上,观察CAM特异性血管生长情况和血管数目变化。结果:氯化胆碱在0.5 nmol/L^1 mmol/L浓度范围内,当浓度增至0.01μmol/L^1 000μmol/L浓度范围时均有促进血管新生的作用,其最低有效浓度为0.01μmol/L,促进血管新生的作用幅度与hVEGF相当。结论:胆碱可促进鸡胚绒毛尿囊膜血管新生。

Objective： To investigate choline promoting angiogenesis on chick embryo chorioallantoic membrane（CAM）.Methods： CAM model was prepared,the choline chloride,human vascular endothelical growth factor（hVEGF） and normal saline were added respectively onto the carrier on the CAM,the state of angiogenesis was observed and the number of new blood vessels was counted.Results： Choline chloride was tested at the concentrations of 0.5 nmol/L～1 mmol/L in this experiment,when its concentrations were increased to 0.01 μmol/L～1 000 μmol/L,it could stimulate angiogenesis,the minimum effective concentration was tested as 0.01 μmol/L, and its effect for promoting the angiogenesis was equivalent to that of hVEGF,the potent stimulator for angiogenesis.Conclusion： The result shows that choline can promote angiogenesis in the chick embryo chorioallantoic membrane.