摘要
目的:探索小剂量过氧化氢预处理与心脏缺血/再灌注损伤之间的关系。方法:SD大鼠随机分为4组,即假手术组、缺血/再灌注组(I/R)、缺血预适应组(IPC)及小剂量过氧化氢预处理组。对各组的心功能与心肌损伤指标进行比较。结果:IPC能够明显保护再灌注损伤,改善心功能。小剂量H2O2预处理能够达到与IPC类似的功效。短暂模拟缺血缺氧导致H2O2产生,小剂量H2O2预处理减少钙超载诱导的细胞色素c释放。结论:IPC导致小剂量H2O2产生,后者通过抑制线粒体通透转换发挥对心肌缺血/再灌注损伤的保护作用。
Objective: To investigate how transient low dose of hydroperoxide pretreatment prevents cardiac ischemia/reperfusion injury.Methods: SD rats were divided into 4 groups:sham operation(Sham),standard ischemia/reperfusion(I/R),ischemic preconditioning(IPC) and IR preceded by low H2O2 treatment.Cardiac function and injury parameter were compared among groups.Results: IPC protected reperfusion injury and improved cardiac function.Low H2O2 treatment played a role in cardioprotection similar to IPC.Low H2O2 was indeed generated in the early phase of simulated ischemia and attenuated cytochrome c release induced by high Ca2+ in isolated mitochondria.Conclusion: Low H2O2 plays a critical role in cardioprotection probably by inhibiting mitochondrial permeability transition.
出处
《中国应用生理学杂志》
CAS
CSCD
2013年第3期247-250,共4页
Chinese Journal of Applied Physiology
基金
天津市自然科学基金(10JCYBJC14800)
国家自然科学基金(81072629)
天津市教委科研计划项目(20100115)
关键词
再灌注损伤
缺血预适应
心肌保护
过氧化氢
reperfusion injury
ischemic preconditioning
cardioprotection
hydroperoxide