运动对动脉粥样硬化大鼠血管内皮细胞AMPK激活的研究

Research about the effect of exercise on the vascular endothelial cells AMPK activated in atherosclerotic rat

目的:研究不同强度运动对大鼠血管内皮细胞的AMP激活蛋白激酶(AMPK)的蛋白表达及其磷酸化的影响,探讨运动改善心血管健康的分子机制。方法:48只Wistar大鼠,分为对照组(C组)、模型组(AS组)、低强度运动模型组(ASL)和高强度运动模型组(ASH)。研究血清白介素-6(IL-6)、肿瘤抑制因子-α(TNF-α)、C反应蛋白(CRP)等炎性因子的浓度,并采用Western blot方法测定大鼠内皮细胞的总AMPK和p-AMPK蛋白量。结果:①AS组IL-6水平与对照组没有显著差异,ASH组则明显升高,与对照组或AS组相比均有显著差异;AS组和ASL组血清TNF-α水平明显高于对照组,但是ASH组和ASL显著低于AS组;AS造模后,各组血清CRP水平均显著高于对照组,各AS造模组间无统计学差异。②ASL和ASH组总AMPK蛋白量明显高于对照组和AS组,与两组相比均有统计学差异。③ASL和ASH组的p-AMPK蛋白均显著高于AS组,ASH组同时显著高于对照组。结论:运动可以降低动脉粥样硬化大鼠的血清炎症因子水平,同时增加血管内皮细胞的AMPK蛋白表达和磷酸化程度。

Objective： To study the molecular mechanism of exercise to improve cardiovascular health,and exploring the effect of different intensity exercise on AMP activated protein kinase（AMPK） protein expression and phosphorylation of vascular endothelial cells in rat.Methods： Forty-eight Wistar rats were divided into control group（group C）,model group（group AS）,low intensity exercise model group（ASL） and high intensity exercise model group（ASH）.The serum inflammatory factor concentrations such as IL-6,TNF-α,CRP were studied,and the endothelial cell total AMPK and p-AMPK protein were checked by means of Western blot. Results： ①The level of IL-6 between AS group and control group had no significant difference,the value of ASH group was markedly increased and had significant difference compared with that of the control group or AS group.The serum TNFα levels of AS group and ASL group were significantly higher than those in control group,but the value of ASH and ASL was significantly lower than that in AS group.The serum concentration of CRP after the AS molding was significantly higher than that of the control group,there was no statistical difference between that of ASL and ASH groups.②The total AMPK protein content of ASL and ASH groups was significantly higher than that in control group.When compared between the two groups,there was astatistical significance.③The value of p-AMPK protein of ASL and ASH groups was significantly higher than that of AS group,the value of p-AMPK protein of ASH group was significantly higher than that of the control group at the same time.Conclusion： Exercise can reduce the serum levels of inflammatory factors in atherosclerosis rat,and increase AMPK protein expression and phosphorylation level in endothelial cell.