运动对高脂饮食大鼠TNF-α、IL-6及脂联素的影响

The effect of exercises on TNF-α,IL-6 and adiponectin in different fat diet rats

目的:探讨运动对不同高脂饮食大鼠血清肿瘤坏死因子-α(TNF-α)、白介素6(IL-6)、脂联素(adiponectin)的影响及运动改善胰岛素抵抗中的相关机制。方法:30只断乳SD雌性大鼠,随机分为三组(n=10):豆油低脂饲料组(LFS)、豆油高脂饲料组(HFS)、豆油高脂饲料合并游泳训练组(HFS+T),共训练8周。观察各组动物体重、体脂百分比、TNF-α、IL-6、脂联素等理化指标变化。结果:与LFS组相比,HFS组大鼠体重、体脂百分比、血清TNF-α、IL-6水平均较高(P<0.05),且血脂联素水平低于LFS组(P<0.05)。经过8周游泳运动的HFS+T组大鼠体重、血清胰岛素、TNF-α均下降,IL-6水平也接近LFS组水平,血清脂联素水平明显升高(P<0.05)。结论:提示运动可能通过增加了脂联素的脂肪动员作用降低内脏脂肪的含量,从而促进糖脂代谢的正常化,改善高脂饮食所导致的胰岛素抵抗。

Objective： To evaluate the role of serum tumor necrosis factor-α（TNF-α） and adiponectin on insulin resistance in different fat diet rats.Methods： Thirty weanling female rats were randomly divided into 3 group（n=10）： a low-fat soybean oil（LFS;22% of total energy fed as fat）,high-fat soybean oil（HFS;40% of total energy fed as fat）,or high-fat soybean oil and swimming training at the same time（HFS＋T）.After fed for 10 weeks,the level of TNF-α,adiponectin in serum of rats were observed.Results： ① The body weight,percentage of body fat of HFS group increased compared with that of LFS group（P0.05）,however those of HFS＋T group were decreased（P0.05）.②The level of serum insulin and ISI in HFS group were increased by LFS group（P0.05）,in HFS＋T group the levels decreased.③And the serum level of TNF-α and IL-6 in HFS group were higher than those in LFS group（P0.05）,the serum levels of adiponectin in HFS group were lower than those in LFS rats,and in HFS＋T group the levels of TNF-α and IL-6 were lower than those in HFS group（P0.05）,the adiponectin level was higher than that in HFS group,and there were no significant difference between LFS group and HFS＋T group.Conclusion： Exercises training could improve sugar and fat metabolism disorders,which also contributes to improving insulin resistance caused by high-fat diet.