靶向磁性纳米颗粒作用鼠胶质瘤动物模型的体内实验研究

Targeted Fe3O4 magnetic multidrug resistance 1 siRNA nanoparticles on the rat BT325 glioblastoma multiform model in vivo

目的观察Fe3O4磁性多药耐药基因（MDR）1siRNA纳米颗粒靶向作用于鼠胶质瘤动物模型体内实验的变化。方法选取20只国际标准化小鼠，建立鼠胶质母细胞瘤BT325动物模型，随机分成实验组和对照组。鼠尾静脉分别注射Fe3O4磁性MDRl siRNA纳米粒子和生理盐水后在外加磁场的靶向作用下，观察其对荷瘤鼠胶质瘤动物模型肿瘤生长的影响，HE普鲁士蓝染色检测靶向结果以及MRI检查铁粒子在肿瘤内分布情况，流式细胞仪检测肿瘤细胞凋亡情况。结果Fe3O4磁性MDRlsiRNA纳米颗粒在外加磁场的靶向作用下，使小鼠胶质母细胞瘤模型中小鼠体质量在第2天时下降最为显著；MRI检测磁性纳米颗粒在T2WI呈高信号，病灶内有铁粒子，肿瘤细胞出现出血坏死表现；HE普鲁士蓝染色，铁粒子呈蓝染；流式细胞仪检测肿瘤细胞荧光定量下降且凋亡峰提前出现。结论Fe3O4磁性MDRlsiRNA纳米颗粒可以抑制胶质瘤的生长，为下一步研究胶质瘤多药耐药的化疗敏感性提供了依据。

Objective To investigate the effect of targeted Fe3O4 magnetic muhidrug resistance （MDR） 1 siRNA nanoparticles on the rat BT325 glioblastoma multiform model. Methods 20 BT325 glioblastoma multiform rat models were build up. They were divided into 2 groups randomly： the Fe3O4 magnetic MDR1 siRNA nanoparticles group and control group. After infusing of the nanoparticles and targeting by the outside magnetic material, the effects were evaluated by the tumor grows and MRI findings. The HE immunocytology of tumor and the FCM were also performed to check the changing of the tumor. Results The rat BT325 glioblastoma multiform model was made successfully. The tumor grew slowly after infusion the F% O4 magnetic MDR1 siRNA nanopartieles and the weight of the rat became less on day 2. The image of MRI showed high signal on T2WI. HE immunoeytology presented Fe particles expression. The FCM demonstrated the apoptosis appeared combined the control group. Conclusions Targeted Fe3O4 magnetic MDR1 siRNA nanopartieles on the rat BT325 glioblastoma multiform model can inhibit tumor growth in vivo and it will increase ehemotherapie sensitivity for glioblastoma muhidrug resistance.