暴发脑膜脑炎流行的新型毒株的全基因序列

Complete Nucleotide Sequence of New Enterovirus Isolated from the Cerebrospinal Fluid of Meningoencephalitis Patients

吉林省延边地区 1996年 6月发生无菌性脑膜脑炎流行 ,从病人脑脊液和粪便标本中分离到多株病毒 ,血清学实验证明所分离的病毒为此次无菌性脑膜脑炎流行的病因。对其中的 2株病毒 (Yanbian96 83csf和Yanbian96 85csf)测定了全基因核酸序列并做了比较分析。结果所测 2株病毒核酸长度均为 745 6bp[包括 3'端poly(A)尾 ],两者间仅有 13个位点不同 ,同源性达 99 8% ,在进化树上位于同一分支 ,因此两者是同一型病毒。通过在GeneBank上检索比较 ,Yanbian96 83csf和所查到的肠道病毒全基因的同源性小于 77% ,结构区VP1同源性大多数小于6 7% ,仅 3株在 83%左右 ;进化树分析 ,与其它肠道病毒相差甚远 ,仅与 2株Echo4和 1株未定型肠道病毒相近 ;而血清学中和试验不能判定为Echo4型。据此推断 ,此病毒为新基因型肠道病毒。有关该病毒结构与功能的研究正在深入进行。

An aseptic meningoencephalitis broke out in Yanbian prefecture of Jilin Province in June, 1996. We isolated 23 virus strains from the cerebrospinal fluid (CSF) and stool specimens of the patients. The isolated viruses could not be typed by neutralization test using the RIVM antiserum provided by WHO. RT PCR showed the viruses were enteroviruses. The serological test demonstrated the isolated virus was the pathogen of the aseptic meningoencephalitis. We determined the complete nucleotide sequences of two strains of the isolated viruses——Yanbian96 83csf and Yanbian96 85csf. The complete nucleotide sequences of Yanbian96 83csf and Yanbian96 85csf both were 7 456 nucleotide long including the 3′ poly(A) tail. The homogeneity of the nucleotide sequence between Yanbian96 83csf and Yanbian96 85csf was 99.8%. In comparison with the sequences of other enteroviruses in GeneBank through internet, the homogeneity of the complete nucleotide sequence was low of 77% and that of the sequence of structural region VP1 was low of 67%. The results demonstrate the isolated viruses belong to new genotype enterovirus.