凝血酶敏感素-2在胃癌中的表达及其临床意义

Clinical significance of thrombospondin-2 expression in gastric carcinoma

目的:探讨胃癌中凝血酶敏感素-2(thrombospondin-2,THBS2)基因表达的临床意义及其与患者临床病理特征的关系,分析THBS2基因表达与胃癌组织中微血管密度(MVD)和基质金属蛋白酶-2(MMP-2)表达水平的相关性。方法:采用实时荧光定量PCR技术检测82例配对的胃癌组织及癌旁组织THBS2 mRNA的表达,并分析其与临床病理特征的关系;应用免疫组织化学Elivision法检测胃癌组织中MMP-2表达情况,通过CD34免疫组织化学染色评价肿瘤组织MVD;分析THBS2 mRNA的表达与MVD、MMP-2表达的相关性。结果:THBS2 mRNA在胃癌中的表达高于癌旁组织(P=0.002);胃癌组织中THBS2 mRNA的表达水平与肿瘤浸润程度、MVD及MMP-2表达密切相关(P=0.023,r=0.35,P<0.01,P=0.004),但与性别、年龄、肿物大小、组织分型及淋巴结转移无关(P=0.53,P=0.53,P=0.21,P=0.84,P=0.96)。结论:THBS2可能在胃癌发生和发展中起重要的作用;THBS2可能通过调节MMP-2的表达,促进肿瘤生长与转移,但其确切的作用及机制有待进一步的研究。

Objective： To investigate the clinical significance ofthrombospondin-2 （THBS2） mRNA expression in gastric carcino- ma and its relationships with clinicopathologic features, microvessel density （MVD）, and matrix metalloproteinase-2 （MMP-2）. Meth- ods： THBS2 mRNA expression was detected in 82 cases of gastric carcinomas and adjacent tissues using real-time quantitative fluores- cence polymerase chain reaction. The correlation of this expression with clinicopathologic features was also analyzed. Cluster of differ- entiation 34 （CD34） and MMP-2 protein expression was examined using an immunohistochemical Elivision method. MVD was deter- mined based on CD34-positive tubular structures. Results： The THBS2 mRNA expression level was significantly higher in the gastric carcinomas than in paraneoplastic tissues （P=0.002）. The expression was associated with the depth of tumor invasion, MVD, and MMP-2 （P=0.02, r= 0.35, P〈0.01, and P=0.004, respectively） but not with patient gender, patient age, tumor size, histological type, and lymph node metastasis （P=0,53, P=0.53, P=0.21, P=0.84, and P=0.96, respectively）. Conclusions： THBS2 may be significantly in- volved in the occurrence and progression of gastric carcinoma. The effects of THBS2 on gastric tumor growth and metastasis can be monitored by controlling the MMP-2 expression in the carcinoma. However, the specific functions and underlying mechanisms of TH- BS2 require further investigation.